LOCUS       sequence-84042-e        1134 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  retroviral expression of human LIN-41 / TRIM71 containing only the 
            NHL repeats and 3 N-terminal HA tags .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1134)
  AUTHORS   Worringer KA, Rand TA, Hayashi Y, Sami S, Takahashi K, Tanabe K, 
            Narita M, Srivastava D, Yamanaka S
  TITLE     The let-7/LIN-41 pathway regulates reprogramming to human induced 
            pluripotent stem cells by controlling expression of 
            prodifferentiation genes.
  JOURNAL   Cell Stem Cell. 2014 Jan 2;14(1):40-52. doi: 
            10.1016/j.stem.2013.11.001. Epub 2013 Nov 14.
  PUBMED    24239284
REFERENCE   2  (bases 1 to 1134)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stem.2013.11.001"; journalName: "Cell Stem Cell"; date: 
            "2014-01-2- 2"; volume: "14"; issue: "1"; pages: "40-52"
COMMENT     Sequence Label: pMXs-hs-3xHA-NHL only-LIN-41
FEATURES             Location/Qualifiers
     source          1..1134
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(883..972)
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     regulatory      975..984
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(1087..1103)
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
ORIGIN
        1 tcgtcctagg aaatgcaatt agaagacgag gattcgattg ttgccaaagt ccaccacaac
       61 gatcattccg tcgggggtga tggcgatgcc ggaagggcgg tccatctgcc caaagccgct
      121 gccttgagca ccaaacttgc acaggaagct gccgttggat tcaaacatct gtacccgatg
      181 gttcctggaa tccgccacaa tgatgcgccc ttcctggtcc acagctaccc cttgtgggcg
      241 caggaactgc ccattgcctg tgccctccga gcccagaaag cgtgccgact ggcagtcggg
      301 gtgaataacc aggagccggt ggttgttgaa gtcagtgacc accaagtggc cctcatggtt
      361 gaaggccaca ccccgtgggg agtcaaagtg cttccagaga gccccctcga agccatactt
      421 gtttaggaag acaccatcag gcccaaacag ctggatccgg tggttcctcg tgtctgagac
      481 caggatcttg ccctcagaat tcaccgccac atcccaaggg tagttgaact gcccattctt
      541 ggttcctttc tcaccaaact tgaggaggaa ctggccctcg aacgtgaaga tctggatgcg
      601 atgattgtcc ttgtcagcca ccacgatcct gcgtgaggcg tcacaggcca cgccggctgg
      661 tcggtcgaac tgcccaggcc gggagcccag ggtgccgaat ttgtggtgga aggcgccgca
      721 gggcttgaac acctggatgc ggttgttgct gcggtcggcg acaatgatgt agccctcctt
      781 gtctacactc acaccccaag ggcggcagag cttgccatcg ctgtcaccct cactgccgaa
      841 gctcaggccc gggagcccaa tgcccacgta gctgcggccc atagcgtaat ctggaacgtc
      901 atatggatag gatcctgcat agtccgggac gtcataggga tagcccgcat agtcaggaac
      961 atcgtatggg tatgccatgg tggcctgcag gaattcccgt accaccacac tgggatcctt
     1021 aattaactag ctagatccgg cagtctagag gatggtccac ccccggggtc ggcagccttc
     1081 acgtgggcgg cgtgtatcca agctgcgatg ccgtctactt tgagggcggt gggg
//
