LOCUS       sequence-84052-e        1010 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  YpdI* is fused to the IFP-F[2] by (GGGGS) X 2. Ypd1*/Skn7t: no 
            signal, Ypd1*/Ssk1t: weak signal. Internal ClaI site in Ypd*I. 
            Plasmid confers ampicillin resistance to DH5-alpha..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1010)
  AUTHORS   Tchekanda E, Sivanesan D, Michnick SW
  TITLE     An infrared reporter to detect spatiotemporal dynamics of 
            protein-protein interactions.
  JOURNAL   Nat Methods. 2014 Jun;11(6):641-4. doi: 10.1038/nmeth.2934. Epub 
            2014 Apr 20.
  PUBMED    24747815
REFERENCE   2  (bases 1 to 1010)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.2934"; journalName: "Nat Methods"; date: "2014-06"; 
            volume: "11"; issue: "6"; pages: "641-4"
COMMENT     Sequence Label: p413GAL1-Ypd*1-L-IFP-F[2] (Yeast)
FEATURES             Location/Qualifiers
     source          1..1010
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     15..35
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        17..35
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        59..500
                     /gene="S. cerevisiae GAL1"
                     /label=GAL1 promoter
                     /note="inducible promoter, regulated by Gal4"
     protein_bind    59..176
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
     primer_bind     471..494
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
ORIGIN
        1 attacgccaa gcgcgcaatt aaccctcact aaagggaaca aaagctggag ctctagtacg
       61 gattagaagc cgccgagcgg gtgacagccc tccgaaggaa gactctcctc cgtgcgtcct
      121 cgtcttcacc ggtcgcgttc ctgaaacgca gatgtgcctc gcgccgcact gctccgaaca
      181 ataaagattc tacaatacta gcttttatgg ttatgaagag gaaaaattgg cagtaacctg
      241 gccccacaaa ccttcaaatg aacgaatcaa attaacaacc ataggatgat aatgcgatta
      301 gttttttagc cttatttctg gggtaattaa tcagcgaagc gatgattttt gatctattaa
      361 cagatatata aatgcaaaaa ctgcataacc actttaacta atactttcaa cattttcggt
      421 ttgtattact tcttattcaa atgtaataaa agtatcaaca aaaaattgtt aatatacctc
      481 tatactttaa cgtcaaggag aaaaaacccc ggattctaga atgtctacta ttccctcaga
      541 aatcatcaat tggaccatct taaatgaaat tatatctatg gatgacgatg attccgattt
      601 ttctaaaggt ctaattattc aatttatcga ccaggcacaa acaacttttg ctcaaatgca
      661 acgacagctg gacggtgaaa aaaatcttac cgaattagac aatctgggcc attttttaaa
      721 gggttcttct gctgcattag gcttacaaag aattgccgcg gtttgtgaaa gaattcaaaa
      781 cttgggaaga aaaatggaac atttcttccc caacaagacc gaattggtca acactctgag
      841 cgataaatcg attattaatg gaatcaatat tgatgaagat gacgaggaaa taaagataca
      901 agtggacgat aaagacgaaa attccatata tctcatcttg atagcaaaag ctttgaacca
      961 gtctaggttg gagttcaaac tggcgagaat tgagttatct aaatattaca
//
