LOCUS       sequence-84056-e         961 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Plasmid template for PCR of cassettes for HR, used to introduce IFP 
            F[2] fragments 3' to the ORF of the genes to study. Plasmid confers 
            ampicillin resistance to DH5-alpha..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 961)
  AUTHORS   Tchekanda E, Sivanesan D, Michnick SW
  TITLE     An infrared reporter to detect spatiotemporal dynamics of 
            protein-protein interactions.
  JOURNAL   Nat Methods. 2014 Jun;11(6):641-4. doi: 10.1038/nmeth.2934. Epub 
            2014 Apr 20.
  PUBMED    24747815
REFERENCE   2  (bases 1 to 961)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.2934"; journalName: "Nat Methods"; date: "2014-06"; 
            volume: "11"; issue: "6"; pages: "641-4"
COMMENT     Sequence Label: pAG32-L-IFP-F[2] (clon 3)
FEATURES             Location/Qualifiers
     source          1..961
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 tggtggcggt ggctctggag gtggtgggtc ctccggagct cgggacagca ttggccctca
       61 cgccctgagg aacgccatgt ttgctctgga aagcgctcca aacctgcggg ctctggccga
      121 agtcgcaaca caaactgtga gagaactgac tggcttcgat cgggtgatgc tgtacaaatt
      181 tgcccctgac gccactggag agatgattgc tgaggccaga cgggagggca tgcaggcttt
      241 tctgggccac aggtttcccg catcccacac ccctgcacaa gctagggccc tctacacaag
      301 acacctgctc cggctgaccg cagacaccag ggctgcagca gtgcccctcg accccgtgct
      361 gaatccccag acaaatgctc ctacacctct gggcggagct gtcctcagag ctacatcccc
      421 aatgcacatg cagtacctga ggaatatggg agtgggctcc tccctgagcg tcagcgtcgt
      481 ggtcggcggc cagctgtggg gactgattgt ctgccaccat cagacaccct acgtgctgcc
      541 accagatctg cggaccaccc tggaggagct ggggagaaag ctgtccgggc aggtgcagag
      601 gaaagaagcc taaggatccg gcggtggcgg atcaggaggc ggtgggtcta tgagtaaagt
      661 agacatggtt tggatagtcg gaggcagttc tgtttaccag gaagccatga atcaaccagg
      721 ccacctcaga ctctttgtga caaggatcat gcaggaattt gaaagtgaca cgtttttccc
      781 agaaattgat ttggggaaat ataaacttct cccagaatac ccaggcgtcc tctctgaggt
      841 ccaggaggaa aaaaggcatc aagtataagt ttgaaagtct acgagaagaa aagactaaat
      901 ctagaaacta gtagctttgg gacttctttc gccagaggtt tggtcaagtc tccaatcaag
      961 g
//
