LOCUS       sequence-84059-e        1403 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  allows Cre-mediated expression of transgene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1403)
  AUTHORS   Nakamura T, Gulick J, Pratt R, Robbins J
  TITLE     Noonan syndrome is associated with enhanced pERK activity, the 
            repression of which can prevent craniofacial malformations.
  JOURNAL   Proc Natl Acad Sci U S A. 2009 Sep 8;106(36):15436-41. doi: 
            10.1073/pnas.0903302106. Epub 2009 Aug 24.
  PUBMED    19706403
REFERENCE   2  (bases 1 to 1403)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.0903302106"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2009-09-8- 8"; volume: "106"; issue: "36"; pages: "15436-41"
COMMENT     Sequence Label: CAG-lox-CAT-lox-unique Bam cloning site-hGH po...
FEATURES             Location/Qualifiers
     source          1..1403
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             126..146
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    571..705
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(608..627)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     662..681
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    complement(727..760)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(977..996)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
ORIGIN
        1 caacctatgg aactgatgaa tgggagcagt ggtggaatgc ctttaatgag gaaaacctgt
       61 tttgctcaga agaaatgcca tctagtgatg atgaggctac tgctgactct caacattcta
      121 ctcctccaaa aaagaagaga aaggtagaag accccaagga ctttccttca gaattgctaa
      181 gttttttgag tcatgctgtg tttagtaata gaactcttgc ttgctttgct atttacacca
      241 caaaggaaaa agctgcactg ctatacaaga aaattatgga aaaatattct gtaaccttta
      301 taagtaggca taacagttat aatcataaca tactgttttt tcttactcca cacaggcata
      361 gagtgtctgc tattaataac tatgctcaaa aattgtgtac ctttagcttt ttaatttgta
      421 aaggggttaa taaggaatat ttgatgtata gtgccttgac tagagatcat aatcagccat
      481 accacatttg tagaggtttt acttgcttta aaaaacctcc cacacctccc cctgaacctg
      541 aaacataaaa tgaatgcaat tgttgttgtt aacttgttta ttgcagctta taatggttac
      601 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
      661 tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggatcgatcc ccggtcgagg
      721 gacctaataa cttcgtatag catacattat acgaagttat attaagggtt ccggatccgg
      781 tggcatccct gtgacccctc cccagtgcct ctcctggccc tggaagttgc cactccagtg
      841 cccaccagcc ttgtcctaat aaaattaagt tgcatcattt tgtctgacta ggtgtccttc
      901 tataatatta tggggtggag gggggtggta tggagcaagg ggcaagttgg gaagacaacc
      961 tgtagggcct gcggggtcta ttgggaacca agctggagtg cagtggcaca atcttggctc
     1021 actgcaatct ccgcctcctg ggttcaagcg attctcctgc ctcagcctcc cgagttgttg
     1081 ggattccagg catgcatgac caggctcagc taatttttgt ttttttggta gagacggggt
     1141 ttcaccatat tggccaggct ggtctccaac tcctaatctc aggtgatcta cccaccttgg
     1201 cctcccaaat tgctgggatt acaggcgtga accactgctc ccttccctgt ccttctgatt
     1261 ttaaaataac tataccagca ggaggacgtc cagacacagc ataggctacc tggccatgcc
     1321 caaccggtgg gacatttgag ttgcttgctt ggcactgtcc tctcatgcgt tgggtccact
     1381 cagtagatgc ctgttgcggc cgc
//
