LOCUS       sequence-84066-e         881 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  genetically encodable fluorescent calcium indicator protein to 
            monitor neuronal activity.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 881)
  AUTHORS   Sun XR, Badura A, Pacheco DA, Lynch LA, Schneider ER, Taylor MP, 
            Hogue IB, Enquist LW, Murthy M, Wang SS
  TITLE     Fast GCaMPs for improved tracking of neuronal activity.
  JOURNAL   Nat Commun. 2013 Jul 17;4:2170. doi: 10.1038/ncomms3170.
  PUBMED    23863808
REFERENCE   2  (bases 1 to 881)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2013-07-17"; volume: "4:2170. doi"; pages: " 
            10.1038/ncomms3170"
COMMENT     Sequence Label: Fast-GCaMP-RS09
FEATURES             Location/Qualifiers
     source          1..881
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(30..47)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(63..506)
                     /codon_start=1
                     /product="calcium-binding protein"
                     /label=calmodulin
                     /translation="DQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEA
                     ELQDMINEVDADGDGTIDFPEFLTMMARKMKDTDSEEEIREAFRVFDKDGNGYISAAEL
                     RHVMTNTGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMTAK"
     primer_bind     622..641
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 tttcgggctt tgttagcagc cggatctcag tggtggtggt ggtggtgctc gagtgcggcc
       61 gctcacttcg ctgtcatcat ttgtacaaac tcttcgtagt ttacctgacc atccccatcg
      121 atgtctgctt ccctgatcat ttcatcaacc tcttcatctg ttaacttctc tccagtgttt
      181 gtcatcacgt ggcgaagctc tgctgcactg atgtagccat tgccatcctt atcaaacaca
      241 cggaacgctt ctctaatttc ttcttcactg tctgtgtctt tcatttttct tgccatcatt
      301 gtcaggaact cagggaagtc gattgtgccg tcaccgtcgg catctacttc attgatcatg
      361 tcctgcagct ctgcttctgt ggggttctgc cccagagacc gcatcaccgt ccccagctcc
      421 ttggttgtta ttgtcccatc cccgtccttg tcaaataggg agaaagcctc tttaaattct
      481 gcgatctgct cttcagtcag ttggtcacgc gtgttgtact ccagcttgtg ccccaggatg
      541 ttgccgtcct ccttgaagtc gatgcccttc agctcgatgc ggttcaccag ggtgtcgccc
      601 tcgaacttca cctcggcgcg ggtcttgtag ttgccgtcgt ccttgaagaa gatggtgcgc
      661 tcctggatgt agccttcggg catggcggac ttgaagaagt cgtgctgctt catgtggtcg
      721 gggtagcggc tgaagcactg cacgccgtag gtcagggtgg tcacgagggt gggccagggc
      781 acgggcagct tgccggtggt gcagatgaac ttcagggtca gcttgccgta ggtggcatcg
      841 ccctcaccct cgccggacac gctgaacttg tggccgttta c
//
