LOCUS       sequence-84068-e        1077 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expression of lacZ driven by human GfaABC1D promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1077)
  AUTHORS   Lee Y, Messing A, Su M, Brenner M
  TITLE     GFAP promoter elements required for region-specific and 
            astrocyte-specific expression.
  JOURNAL   Glia. 2008 Apr;56(5):481-93. doi: 10.1002/glia.20622.
  PUBMED    18240313
REFERENCE   2  (bases 1 to 1077)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/glia"; 
            journalName: "Glia"; date: "2008-04"; volume: "56"; issue: "5"; 
            pages: "481-93"
COMMENT     Sequence Label: pGfaABC1D-nLac
FEATURES             Location/Qualifiers
     source          1..1077
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             767..787
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(808..830)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 cgaattcgag ctcggtacca gatctaacat atcctggtgt ggagtagggg acgctgctct
       61 gacagaggct cgggggcctg agctggctct gtgagctggg gaggaggcag acagccaggc
      121 cttgtctgca agcagacctg gcagcattgg gctggccgcc ccccagggcc tcctcttcat
      181 gcccagtgaa tgactcacct tggcacagac acaatgttcg gggtgggcac agtgcctgct
      241 tcccgccgca ccccagcccc cctcaaatgc cttccgagaa gcccattgag cagggggctt
      301 gcattgcacc ccagcctgac agcctggcat cttgggataa aagcagcaca gccccctagg
      361 ggctgccctt gctgtgtggc gccaccggcg gtggagaaca aggctctatt cagcctgtgc
      421 ccaggaaagg ggatcagggg atgcccaggc atggacagtg ggtggcaggg ggggagagga
      481 gggctgtctg cttcccagaa gtccaaggac acaaatgggt gaggggagag ctctccccat
      541 agctgggctg cggcccaacc ccaccccctc aggctatgcc agggggtgtt gccaggggca
      601 cccgggcatc gccagtctag cccactcctt cataaagccc tcgcatccca ggagcgagca
      661 gagccagagc aggttggaga ggagacgcat cacctccgct gctcgcgggg atcctctaga
      721 gtcgacggat ccggggaatt ccccagtctc aggatccacc atggggccca agaagaaacg
      781 caaagtgggg agcatggggg atcccgtcgt tttacaacgt cgtgactggg aaaaccctgg
      841 cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga
      901 agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatggcgctt
      961 tgcctggttt ccggcaccag aagcggtgcc ggaaagctgg ctggagtgcg atcttcctga
     1021 ggccgatact gtcgtcgtcc cctcaaactg gcagatgcac ggttacgatg cgcccat
//
