LOCUS       sequence-84070-e         969 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expression of lacZ driven by human GfaABC1D promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 969)
  AUTHORS   Lee Y, Messing A, Su M, Brenner M
  TITLE     GFAP promoter elements required for region-specific and 
            astrocyte-specific expression.
  JOURNAL   Glia. 2008 Apr;56(5):481-93. doi: 10.1002/glia.20622.
  PUBMED    18240313
REFERENCE   2  (bases 1 to 969)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/glia"; 
            journalName: "Glia"; date: "2008-04"; volume: "56"; issue: "5"; 
            pages: "481-93"
COMMENT     Sequence Label: pGfaABC1D-nLac
FEATURES             Location/Qualifiers
     source          1..969
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     165..187
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     CDS             complement(208..228)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 ttccggcacc gcttctggtg ccggaaacca ggcaaagcgc cattcgccat tcaggctgcg
       61 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      121 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      181 taaaacgacg ggatccccca tgctccccac tttgcgtttc ttcttgggcc ccatggtgga
      241 tcctgagact ggggaattcc ccggatccgt cgactctaga ggatccccgc gagcagcgga
      301 ggtgatgcgt ctcctctcca acctgctctg gctctgctcg ctcctgggat gcgagggctt
      361 tatgaaggag tgggctagac tggcgatgcc cgggtgcccc tggcaacacc ccctggcata
      421 gcctgagggg gtggggttgg gccgcagccc agctatgggg agagctctcc cctcacccat
      481 ttgtgtcctt ggacttctgg gaagcagaca gccctcctct ccccccctgc cacccactgt
      541 ccatgcctgg gcatcccctg atcccctttc ctgggcacag gctgaataga gccttgttct
      601 ccaccgccgg tggcgccaca cagcaagggc agcccctagg gggctgtgct gcttttatcc
      661 caagatgcca ggctgtcagg ctggggtgca atgcaagccc cctgctcaat gggcttctcg
      721 gaaggcattt gaggggggct ggggtgcggc gggaagcagg cactgtgccc accccgaaca
      781 ttgtgtctgt gccaaggtga gtcattcact gggcatgaag aggaggccct ggggggcggc
      841 cagcccaatg ctgccaggtc tgcttgcaga caaggcctgg ctgtctgcct cctccccagc
      901 tcacagagcc agctcaggcc cccgagcctc tgtcagagca gcgtccccta ctccacacca
      961 ggatatgtt
//
