LOCUS       sequence-84074-e         992 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expression of nuclear LacZ driven by Gfa2 promoter with 3 additional
            B sites, has about 75 times higher activity than pGfa2-nLac in 
            transfected cells .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 992)
  AUTHORS   de Leeuw B, Su M, ter Horst M, Iwata S, Rodijk M, Hoeben RC, Messing
            A, Smitt PS, Brenner M
  TITLE     Increased glia-specific transgene expression with glial fibrillary 
            acidic protein promoters containing multiple enhancer elements.
  JOURNAL   J Neurosci Res. 2006 Apr;83(5):744-53.
  PUBMED    16496373
REFERENCE   2  (bases 1 to 992)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Neurosci 
            Res."; date: "2006-04"; volume: "83(5)"; pages: "744-53"
COMMENT     Sequence Label: pGfa(B3)-nLac
FEATURES             Location/Qualifiers
     source          1..992
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     156..178
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     CDS             complement(199..219)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 cgcttctggt gccggaaacc aggcaaagcg ccattcgcca ttcaggctgc gcaactgttg
       61 ggaagggcga tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc
      121 tgcaaggcga ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac
      181 gggatccccc atgctcccca ctttgcgttt cttcttgggc cccatggtgg atcctgagac
      241 tggggaattc cccggatccg tcgactctag aggatccccg cgagcagcgg aggtgatgcg
      301 tctcctctcc aacctgctct ggctctgctc gctcctggga tgcgagggct ttatgaagga
      361 gtgggctaga ctggcgatgc ccgggcctgc tcaatgggct tctcggaagg catttgaggg
      421 gggctggggt gcggcgggaa gcaggcactg tgcccacccc gaacattgtg tctgtgccaa
      481 ggtgagtcat tcactgggca tgaagaggaa gatcgggcct gctcaatggg cttctcggaa
      541 ggcatttgag gggggctggg gtgcggcggg aagcaggcac tgtgcccacc ccgaacattg
      601 tgtctgtgcc aaggtgagtc attcactggg catgaagagg aagatcgggc ctgctcaatg
      661 ggcttctcgg aaggcatttg aggggggctg gggtgcggcg ggaagcaggc actgtgccca
      721 ccccgaacat tgtgtctgtg ccaaggtgag tcattcactg ggcatgaaga ggaagatcgg
      781 gtgcccctgg caacaccccc tggcatagcc tgagggggtg gggttgggcc gcagcccagc
      841 tatggggaga gctcccctca ccccactgag gtcactgtgc ccagaggcag agcttcctgg
      901 gcacgccaac tcctgcatga ggcaggtggt acctggagcc agaccatggg tctggggccc
      961 tgaccgctga agcaagagga ccctctctct ag
//
