LOCUS       sequence-84075-e       12248 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Contains crtE, crtB, and crtI genes of Erwinia herbicola (Pantoea 
            agglomerans) Eho10, together with the lcyE gene of Arabidopsis 
            thaliana and thereby produces delta-carotene in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12248)
  AUTHORS   Sun Z, Gantt E, Cunningham FX Jr
  TITLE     Cloning and functional analysis of the beta-carotene hydroxylase of 
            Arabidopsis thaliana.
  JOURNAL   J Biol Chem. 1996 Oct 4;271(40):24349-52.
  PUBMED    8798688
REFERENCE   2  (bases 1 to 12248)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "1996-10-4"; volume: "271(40)"; pages: "24349-52"
COMMENT     Sequence Label: pAC-DELTA
FEATURES             Location/Qualifiers
     source          1..12248
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     134..153
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(220..322)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     complement(714..731)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(848..1393)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(928..947)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        join(2042..2047,2048..2064,2065..2071)
                     /label=trc promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    2079..2095
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2084..2106
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     misc_feature    2112..2181
                     /label=rrnG antiterminator
                     /note="antiterminator from the E. coli rrnG leader region 
                     (Berg et al., 1989)"
     CDS             2232..2258
                     /codon_start=1
                     /product="synthetic cistron containing a ribosome binding 
                     site (Shine-Dalgarno sequence), for enhancing the bacterial
                     expression of a downstream cistron (Schoner, 1997)"
                     /label=minicistron
                     /note="This first cistron should terminate 3 bp upstream of
                     the ATG for the second cistron."
                     /translation="MYRLNKEE"
     CDS             2274..2291
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             2295..2327
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             2340..2354
                     /codon_start=1
                     /product="enterokinase recognition and cleavage site"
                     /label=enterokinase site
                     /translation="DDDDK"
     primer_bind     complement(9936..9955)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     CDS             complement(join(11808..12248,1..219))
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
ORIGIN
        1 gaattccgga tgagcattca tcaggcgggc aagaatgtga ataaaggccg gataaaactt
       61 gtgcttattt ttctttacgg tctttaaaaa ggccgtaata tccagctgaa cggtctggtt
      121 ataggtacat tgagcaactg actgaaatgc ctcaaaatgt tctttacgat gccattggga
      181 tatatcaacg gtggtatatc cagtgatttt tttctccatt ttagcttcct tagctcctga
      241 aaatctcgat aactcaaaaa atacgcccgg tagtgatctt atttcattat ggtgaaagtt
      301 ggaacctctt acgtgccgat caacgtctca ttttcgccaa aagttggccc agggcttccc
      361 ggtatcaaca gggacaccag gatttattta ttctgcgaag tgatcttccg tcacaggtat
      421 ttattcggcg caaagtgcgt cgggtgatgc tgccaactta ctgatttagt gtatgatggt
      481 gtttttgagg tgctccagtg gcttctgttt ctatcagctg tccctcctgt tcagctactg
      541 acggggtggt gcgtaacggc aaaagcaccg ccggacatca gcgctagcgg agtgtatact
      601 ggcttactat gttggcactg atgagggtgt cagtgaagtg cttcatgtgg caggagaaaa
      661 aaggctgcac cggtgcgtca gcagaatatg tgatacagga tatattccgc ttcctcgctc
      721 actgactcgc tacgctcggt cgttcgactg cggcgagcgg aaatggctta cgaacggggc
      781 ggagatttcc tggaagatgc caggaagata cttaacaggg aagtgagagg gccgcggcaa
      841 agccgttttt ccataggctc cgcccccctg acaagcatca cgaaatctga cgctcaaatc
      901 agtggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggcggctccc
      961 tcgtgcgctc tcctgttcct gcctttcggt ttaccggtgt cattccgctg ttatggccgc
     1021 gtttgtctca ttccacgcct gacactcagt tccgggtagg cagttcgctc caagctggac
     1081 tgtatgcacg aaccccccgt tcagtccgac cgctgcgcct tatccggtaa ctatcgtctt
     1141 gagtccaacc cggaaagaca tgcaaaagca ccactggcag cagccactgg taattgattt
     1201 agaggagtta gtcttgaagt catgcgccgg ttaaggctaa actgaaagga caagttttgg
     1261 tgactgcgct cctccaagcc agttacctcg gttcaaagag ttggtagctc agagaacctt
     1321 cgaaaaaccg ccctgcaagg cggttttttc gttttcagag caagagatta cgcgcagacc
     1381 aaaacgatct caagaagatc atcttattaa tcagataaaa tatttctaga tttcagtgca
     1441 atttatctct tcaaatgtag cacctgaagt cagccccata cgatataagt tgtaattctc
     1501 atgtttgaca gcttatcatc gataagctat ctcggtagtg ggatacgacg ataccgaaga
     1561 cagctcatgt tatatcccgc cgtcaaccac catcaaacag gattttcgcc tgctggggca
     1621 aaccagcgtg gaccgcttgc tgcaactctc tcagggccag gcggtgaagg gcaatcagct
     1681 gttgcccgtc tcactggtga aaagaaaaac caccctggcg cccaatacgc aaaccgcctc
     1741 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag
     1801 cgggcagtga gcgcaacgca attaatgtga gttagcgcga attgatctgg tttgacagct
     1861 tatcatcgac tgcacggtgc accaatgctt ctggcgtcag gcagccatcg gaagctgtgg
     1921 tatggctgtg caggtcgtaa atcactgcat aattcgtgtc gctcaaggcg cactcccgtt
     1981 ctggataatg ttttttgcgc cgacatcata acggttctgg caaatattct gaaatgagct
     2041 gttgacaatt aatcatccgg ctcgtataat gtgtggaatt gtgagcggat aacaatttca
     2101 cacaggaaac agcgccgctg agaaaaagcg aagcggcact gctctttaac aatttatcag
     2161 acaatctgtg tgggcactcg accggaatta tcgattaact ttattattaa aaattaaaga
     2221 ggtatatatt aatgtatcga ttaaataagg aggaataaac catggggggt tctcatcatc
     2281 atcatcatca tggtatggct agcatgactg gtggacagca aatgggtcgg actctgtacg
     2341 acgatgacga taaggatcga tggggatcat cgaattcgcg gccgctacaa aaggaaataa
     2401 ttagattcct ctttctgctt gctatacctt gatagaacaa tataacaatg gtgtaagtct
     2461 tctcgctgta ttcgaaatta tttggaggag gaaaatggag tgtgttgggg ctaggaattt
     2521 cgcagcaatg gcggtttcaa catttccgtc atggagttgt cgaaggaaat ttccagtggt
     2581 taagagatac agctatagga atattcgttt cggtttgtgt agtgtcagag ctagcggcgg
     2641 cggaagttcc ggtagtgaga gttgtgtagc ggtgagagaa gatttcgctg acgaagaaga
     2701 ttttgtgaaa gctggtggtt ctgagattct atttgttcaa atgcagcaga acaaagatat
     2761 ggatgaacag tctaagcttg ttgataagtt gcctcctata tcaattggtg atggtgcttt
     2821 ggatcatgtg gttattggtt gtggtcctgc tggtttagcc ttggctgcag aatcagctaa
     2881 gcttggatta aaagttggac tcattggtcc agatcttcct tttactaaca attacggtgt
     2941 ttgggaagat gaattcaatg atcttgggct gcaaaaatgt attgagcatg tttggagaga
     3001 gactattgtg tatctggatg atgacaagcc tattaccatt ggccgtgctt atggaagagt
     3061 tagtcgacgt ttgctccatg aggagctttt gaggaggtgt gtcgagtcag gtgtctcgta
     3121 ccttagctcg aaagttgaca gcataacaga agcttctgat ggccttagac ttgttgcttg
     3181 tgacgacaat aacgtcattc cctgcaggct tgccactgtt gcttctggag cagcttcggg
     3241 aaagctcttg caatacgaag ttggtggacc tagagtctgt gtgcaaactg catacggcgt
     3301 ggaggttgag gtggaaaata gtccatatga tccagatcaa atggttttca tggattacag
     3361 agattatact aacgagaaag ttcggagctt agaagctgag tatccaacgt ttctgtacgc
     3421 catgcctatg acaaagtcaa gactcttctt cgaggagaca tgtttggcct caaaagatgt
     3481 catgcccttt gatttgctaa aaacgaagct catgttaaga ttagatacac tcggaattcg
     3541 aattctaaag acttacgaag aggagtggtc ctatatccca gttggtggtt ccttgccaaa
     3601 caccgaacaa aagaatctcg cctttggtgc tgccgctagc atggtacatc ccgcaacagg
     3661 ctattcagtt gtgagatctt tgtctgaagc tccaaaatat gcatcagtca tcgcagagat
     3721 actaagagaa gagactacca aacagatcaa cagtaatatt tcaagacaag cttgggatac
     3781 tttatggcca ccagaaagga aaagacagag agcattcttt ctctttggtc ttgcactcat
     3841 agttcaattc gataccgaag gcattagaag cttcttccgt actttcttcc gccttccaaa
     3901 atggatgtgg caagggtttc taggatcaac attaacatca ggagatctcg ttctctttgc
     3961 tttatacatg ttcgtcattt caccaaacaa tttgagaaaa ggtctcatca atcatctcat
     4021 ctctgatcca accggagcaa ccatgataaa aacctatctc aaagtatgat ttacttatca
     4081 actcttaggt ttgtgtatat atatgttgat ttatctgaat aatcgatcaa agaatggtat
     4141 gtgggttact aggaagttgg aaacaaacat gtatagaatc taaggagtga tcgaaatgga
     4201 gatggaaacg aaaagaaaaa aatcagtctt tgttttgtgg ttagtgacgc ggccgcgaat
     4261 tcctgcagcc cgatccgagc tcgagatctg cagctgagct ttaatgcggt agtttatcac
     4321 agttaaattg ctaacgcagt caggcaccgt gtatgaaatc taacaatgcg ctcatcgtca
     4381 tcctcggcac cgtcaccctg gatgctgtag gcataggctt ggttatgccg gtactgccgg
     4441 gcctcttgcg ggatatcgtc cattccgaca gcatcgccag tcactatggc gtgctgctag
     4501 cgctatatgc gttgatgcaa tttctatgcg cacccgttct cggagcactg tccgaccgct
     4561 ttggccgccg cccagtcctg ctcgcttcgc tacttggagc cactatcgac tacgcgatca
     4621 tggcgaccac acccgtcctg tggatctaaa ggcacagcgt ctcatgcttc gcacaatgta
     4681 aaactgcttc agaacctggc gagagctatc cgcgcggtct acggttaact gatactaaaa
     4741 gacaattcag cgggtaacct tgcaatggtg agtggcagta aagcgggcgt ttcgcctcat
     4801 cgcgaaatag aagtaatgag acaatccatt gacgatcacc tggctggcct gttacctgaa
     4861 accgacagcc aggatatcgt cagccttgcg atgcgtgaag gcgtcatggc acccggtaaa
     4921 cggatccgtc cgctgctgat gctgctggcc gcccgcgacc tccgctacca gggcagtatg
     4981 cctacgctgc tcgatctcgc ctgcgccgtt gaactgaccc ataccgcgtc gctgatgctc
     5041 gacgacatgc cctgcatgga caacgccgag ctgcgccgcg gtcagcccac tacccacaaa
     5101 aaatttggtg agagcgtggc gatccttgcc tccgttgggc tgctctctaa agcctttggt
     5161 ctgatcgccg ccaccggcga tctgccgggg gagaggcgtg cccaggcggt caacgagctc
     5221 tctaccgccg tgggcgtgca gggcctggta ctggggcagt ttcgcgatct taacgatgcc
     5281 gccctcgacc gtacccctga cgctatcctc agcaccaacc acctcaagac cggcattctg
     5341 ttcagcgcga tgctgcagat cgtcgccatt gcttccgcct cgtcgccgag cacgcgagag
     5401 acgctgcacg ccttcgccct cgacttcggc caggcgtttc aactgctgga cgatctgcgt
     5461 gacgatcacc cggaaaccgg taaagatcgc aataaggacg cgggaaaatc gacgctggtc
     5521 aaccggctgg gcgcagacgc ggcccggcaa aagctgcgcg agcatattga ttccgccgac
     5581 aaacacctca cttttgcctg tccgcagggc ggcgccatcc gacagtttat gcatctgtgg
     5641 tttggccatc accttgccga ctggtcaccg gtcatgaaaa tcgcctgata ccgccctttt
     5701 gggttcaagc agtacataac gatggaacca cattacagga gtagtgatga atgaaggacg
     5761 agcgccttgt tcagcgtaag aacgatcatc tggatatcgt tctcgacccc cgtcgcgccg
     5821 taactcaggc tagcgcaggt tttgagcgct ggcgctttac ccactgcgcc ctgccagagc
     5881 tgaattttag cgacatcacg ctggaaacca ccttcctgaa tcggcagcta caggctccgc
     5941 tgctgatcag ctccatgacc ggcggcgttg agcgctcgcg ccatatcaac cgccacctcg
     6001 ccgaggcggc gcaggtgcta aaaattgcga tgggggtggg ctcccagcgc gtcgccattg
     6061 agagcgacgc gggcttaggg ctggataaaa ccctgcggca gctggctccg gacgtgccgc
     6121 tgctggcgaa cctcggcgcg gcgcagctga ccggcagaaa aggtattgat tacgcccgac
     6181 gggccgtgga gatgatcgag gcggatgcgc tgattgtgca cctaaacccg ctgcaggagg
     6241 cgctacagcc cggcggcgat cgcgactggc gcggacggct ggcggctatt gaaactctgg
     6301 tccgcgagct gcccgttccg ctggtggtga aagaggtggg agccggtatc tcccgaaccg
     6361 tggccgggca gctgatcgat gccggcgtta ccgtgattga cgtcgcgggc gcgggcggca
     6421 ccagctgggc cgccgttgaa ggcgagcggg cggccaccga gcagcagcgc agcgtggcca
     6481 acgtctttgc cgactggggg atccccaccg ctgaggcgct ggttgacatt gccgaggcct
     6541 ggccgcagat gccccttatt gcctcgggaa tgcgggctgg gctatttcac cctaccactg
     6601 gctattcgct gccgctggcg gtggcccttg ccgacgcgat tgccgacagc ccgcggctgg
     6661 gcagcgttcc gctctatcag ctcacccggc agtttgccga acgccactgg cgcaggcagg
     6721 gattcttccg cctgctgaac cggatgcttt tcctggccgg gcgcgaggag aaccgctggc
     6781 gggtgatgca gcgcttttat gggctgccgg agcccaccgt agagcgcttt tacgccggtc
     6841 ggctctctct ctttgataag gcccgcattt tgacgggcaa gccaccggtt ccgctgggcg
     6901 aagcctggcg ggcggcgctg aaccattttc ctgacagacg agataaagga tgaaaaaaac
     6961 cgttgtgatt ggcgcaggct ttggtggcct ggcgctggcg attcgcctgc aggcggcagg
     7021 gatcccaacc gtactgctgg agcagcggga caagcccggc ggtcgggcct acgtctggca
     7081 tgaccagggc tttacctttg acgccgggcc gacggtgatc accgatccta ccgcgcttga
     7141 ggcgctgttc accctggccg gcaggcgcat ggaggattac gtcaggctgc tgccggtaaa
     7201 acccttctac cgactctgct gggagtccgg gaagaccctc gactatgcta acgacagcgc
     7261 cgagcttgag gcgcagatta cccagttcaa cccccgcgac gtcgagggct accggcgctt
     7321 tctggcttac tcccaggcgg tattccagga gggatatttg cgcctcggca gcgtgccgtt
     7381 cctctctttt cgcgacatgc tgcgcgccgg gccgcagctg cttaagctcc aggcgtggca
     7441 gagcgtctac cagtcggttt cgcgctttat tgaggatgag catctgcggc aggccttctc
     7501 gttccactcc ctgctggtag gcggcaaccc cttcaccacc tcgtccatct acaccctgat
     7561 ccacgccctt gagcgggagt ggggggtctg gttccctgag ggcggcaccg gggcgctggt
     7621 gaacggcatg gtgaagctgt ttaccgatct gggcggggag atcgaactca acgcccgggt
     7681 cgaagagctg gtggtggccg ataaccgcgt aagccaggtc cggctggcgg atggtcggat
     7741 ctttgacacc gacgccgtag cctcgaacgc tgacgtggtg aacacctata aaaagctgct
     7801 cggccaccat ccggtggggc agaagcgggc ggcagcgctg gagcgcaaga gcatgagcaa
     7861 ctcgctgttt gtgctctact tcggcctgaa ccagcctcat tcccagctgg cgcaccatac
     7921 catctgtttt ggtccccgct accgggagct gatcgacgag atctttaccg gcagcgcgct
     7981 ggcggatgac ttctcgctct acctgcactc gccctgcgtg accgatccct cgctcgcgcc
     8041 tcccggctgc gccagcttct acgtgctggc cccggtgccg catcttggca acgcgccgct
     8101 ggactgggcg caggaggggc cgaagctgcg cgaccgcatc tttgactacc ttgaagagcg
     8161 ctatatgccc ggcctgcgta gccagctggt gacccagcgg atctttaccc cggcagactt
     8221 ccacgacacg ctggatgcgc atctgggatc ggccttctcc atcgagccgc tgctgaccca
     8281 aagcgcctgg ttccgcccgc acaaccgcga cagcgacatt gccaacctct acctggtggg
     8341 cgcaggtact caccctgggg cgggcattcc tggcgtagtg gcctcggcga aagccaccgc
     8401 cagcctgatg attgaggatc tgcaatgagc caaccgccgc tgcttgacca cgccacgcag
     8461 accatggcca acggctcgaa aagttttgcc accgctgcga agctgttcga cccggccacc
     8521 cgccgtagcg tgctgatgct ctacacctgg tgccgccact gcgatgacgt cattgacgac
     8581 cagacccacg gcttcgccag cgaggccgcg gcggaggagg aggccaccca gcgcctggcc
     8641 cggctgcgca cgctgaccct ggcggcgttt gaaggggccg agatgcagga tccggccttc
     8701 gctgcctttc aggaggtggc gctgacccac ggtattacgc cccgcatggc gctcgatcac
     8761 ctcgacggct ttgcgatgga cgtggctcag acccgctatg tcacctttga ggatacgctg
     8821 cgctactgct atcacgtggc gggcgtggtg ggtctgatga tggccagggt gatgggcgtg
     8881 cgggatgagc gggtgctgga tcgcgcctgc gatctggggc tggccttcca gctgacgaat
     8941 atcgcccggg atattattga cgatgcggct attgaccgct gctatctgcc cgccgagtgg
     9001 ctgcaggatg ccgggctgac cccggagaac tatgccgcgc gggagaatcg ggccgcgctg
     9061 gcgcgggtgg cggagcggct tattgatgcc gcagagccgt actacatctc ctcccaggcc
     9121 gggctacacg atctgccgcc gcgctgcgcc tgggcgatcg ccaccgcccg cagcgtctac
     9181 cgggagatcg gtattaaggt aaaagcggcg ggaggcagcg cctgggatcg ccgccagcac
     9241 accagcaaag gtgaaaaaat tgccatgctg atggcggcac cggggcaggt tattcgggcg
     9301 aagacgacga gggtgacgcc gcgtccggcc ggtctttggc agcgtcccgt ttaggcgggc
     9361 ggccatgacg ttcacgcagg atcgcctgta ggtcggcagg cttgcgggcg taaataaaac
     9421 cgaaggagac gcagccctcc cggccgcgca ccgcgtggtg caggcggtgg gcgacgtaga
     9481 gccgcttcag gtagccccgg cgcgggatcc cgcctcgccg tgctgtccgg tctcaacctg
     9541 atccgccaga atcgagccaa cgggatcggc cagcacgaat tcggtatgcg gagagtgttt
     9601 ggcaaaccat gcctgcaagc cacccagcgt gccgccggag ccaacgccta ctaccacggc
     9661 atcaaccctg cctgccagct ggtcgaacag ctccggtgcg gtggtggtgg cgtgcgccag
     9721 cgggttggcc gggttagaga actgatcaat atagtaagca cccggcgtct cctctgccag
     9781 gcggcgggcg tagtcctgat agtactccgg gtggcccttt gtcacgtcgg agcgggtcag
     9841 gcgcacatca acacccagcg cacgcaggtg gtagatcctc tacgccggac gcatcgtggc
     9901 cggcatcacc ggcgccacag gtgcggttgc tggcgcctat atcgccgaca tcaccgatgg
     9961 ggaagatcgg gctcgccact tcgggctcat gagcgcttgt ttcggcgtgg gtatggtggc
    10021 aggccccgtg gccgggggac tgttgggcgc catctccttg catgcaccat tccttgcggc
    10081 ggcggtgctc aacggcctca acctactact gggctgcttc ctaatgcagg agtcgcataa
    10141 gggagagcgt cgaccgatgc ccttgagagc cttcaaccca gtcagctcct tccggtgggc
    10201 gcggggcatg actatcgtcg ccgcacttat gactgtcttc tttatcatgc aactcgtagg
    10261 acaggtgccg gcagcgctct gggtcatttt cggcgaggac cgctttcgct ggagcgcgac
    10321 gatgatcggc ctgtcgcttg cggtattcgg aatcttgcac gccctcgctc aagccttcgt
    10381 cactggtccc gccaccaaac gtttcggcga gaagcaggcc attatcgccg gcatggcggc
    10441 cgacgcgctg ggctacgtct tgctggcgtt cgcgacgcga ggctggatgg ccttccccat
    10501 tatgattctt ctcgcttccg gcggcatcgg gatgcccgcg ttgcaggcca tgctgtccag
    10561 gcaggtagat gacgaccatc agggacagct tcaaggatcg ctcgcggctc ttaccagcct
    10621 aacttcgatc actggaccgc tgatcgtcac ggcgatttat gccgcctcgg cgagcacatg
    10681 gaacgggttg gcatggattg taggcgccgc cctatacctt gtctgcctcc ccgcgttgcg
    10741 tcgcggtgca tggagccggg ccacctcgac ctgaatggaa gccggcggca cctcgctaac
    10801 ggattcacca ctccaagaat tggagccaat caattcttgc ggagaactgt gaatgcgcaa
    10861 accaaccctt ggcagaacat atccatcgcg tccgccatct ccagcagccg cacgcggcgc
    10921 atctcgggca gcgttgggtc ctggccacgg gtgcgcatga tcgtgctcct gtcgttgagg
    10981 acccggctag gctggcgggg ttgccttact ggttagcaga atgaatcacc gatacgcgag
    11041 cgaacgtgaa gcgactgctg ctgcaaaacg tctgcgacct gagcaacaac atgaatggtc
    11101 ttcggtttcc gtgtttcgta aagtctggaa acgcggaagt cccctacgtg ctgctgaagt
    11161 tgcccgcaac agagagtgga accaaccggt gataccacga tactatgact gagagtcaac
    11221 gccatgagcg gcctcatttc ttattctgag ttacaacagt ccgcaccgct gtccggtagc
    11281 tccttccggt gggcgcgggg catgactatc gtcgccgcac ttatgactgt cttctttatc
    11341 atgcaactcg taggacaggt gccggcagcg cccaacagtc ccccggccac ggggcctgcc
    11401 accataccca cgccgaaaca agcgccctgc accattatgt tccggatctg catcgcagga
    11461 tgctgctggc taccctgtgg aacacctaca tctgtattaa cgaagcgcta accgttttta
    11521 tcaggctctg ggaggcagaa taaatgatca tatcgtcaat tattacctcc acggggagag
    11581 cctgagcaaa ctggcctcag gcatttgaga agcacacggt cacactgctt ccggtagtca
    11641 ataaaccggt aaaccagcaa tagacataag cggctattta acgaccctgc cctgaaccga
    11701 cgaccgggtc gaatttgctt tcgaatttct gccattcatc cgcttattat cacttattca
    11761 ggcgtagcac caggcgttta agggcaccaa taactgcctt aaaaaaatta cgccccgccc
    11821 tgccactcat cgcagtactg ttgtaattca ttaagcattc tgccgacatg gaagccatca
    11881 cagacggcat gatgaacctg aatcgccagc ggcatcagca ccttgtcgcc ttgcgtataa
    11941 tatttgccca tggtgaaaac gggggcgaag aagttgtcca tattggccac gtttaaatca
    12001 aaactggtga aactcaccca gggattggct gagacgaaaa acatattctc aataaaccct
    12061 ttagggaaat aggccaggtt ttcaccgtaa cacgccacat cttgcgaata tatgtgtaga
    12121 aactgccgga aatcgtcgtg gtattcactc cagagcgatg aaaacgtttc agtttgctca
    12181 tggaaaacgg tgtaacaagg gtgaacacta tcccatatca ccagctcacc gtctttcatt
    12241 gccatacg
//
