LOCUS       sequence-84084-e        8715 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Bidirectional construct in the pcDNA5/FRT backbone with GFP and 
            mCherry driven by the same CMV promoter along with a Gateway cloning
            site downstream mCherry.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8715)
  AUTHORS   Oikonomou P, Goodarzi H, Tavazoie S
  TITLE     Systematic Identification of Regulatory Elements in Conserved 3' 
            UTRs of Human Transcripts.
  JOURNAL   Cell Rep. 2014 Apr 10;7(1):281-92. doi: 
            10.1016/j.celrep.2014.03.001. Epub 2014 Mar 20.
  PUBMED    24656821
REFERENCE   2  (bases 1 to 8715)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2014.03.001"; journalName: "Cell Rep"; date: 
            "2014-04-10- 10"; volume: "7"; issue: "1"; pages: "281-92"
COMMENT     Sequence Label: pcDNA5-FRT-GFP-mCherry-3pGW
FEATURES             Location/Qualifiers
     source          1..8715
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     polyA_signal    266..347
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(486..1205)
                     /codon_start=1
                     /product="Aequorea coerulescens GFP"
                     /label=AcGFP1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGAELFTGIVPILIELNGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSYGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFEDD
                     GNYKSRAEVKFEGDTLVNRIELTGTDFKEDGNILGNKMEYNYNAHNVYIMTDKAKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMIYF
                     GFVTAAAITHGMDELYK"
     regulatory      1202..1211
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     enhancer        1302..1681
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1682..1885
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1836..1856
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    1887..1905
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1908..1926
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     regulatory      2057..2066
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2063..2773
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(2208..2226)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     2476..2495
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     protein_bind    2805..2929
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(2955..2960,2961..2978,2979..2985)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             3039..3698
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(3105..3124)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             4040..4345
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     4224..4243
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(4386..4510)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(4667..4684)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4673..4897
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(5030..5049)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    5181..5228
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             5236..6258
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             5236..6258
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    6388..6509
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     6479..6498
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6558..6574)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6558..6574)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6571..6593)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6582..6598
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6606..6612,6613..6630,6631..6636))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6651..6672
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6789..6806)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6960..7548)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7040..7059)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7719..8510,8511..8579))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8342..8361
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8580..8684)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgtcacattt
      241 ccccgaaaag tgccacctga cgtcggcagt gaaaaaaatg ctttatttgt gaaatttgtg
      301 atgctattgc tttatttgta accattataa gctgcaataa acaagttaac aacaacaatt
      361 gcattcattt tatgtttcag gttcaggggg aggtgtggga ggttttttaa agcaagtaaa
      421 acctctacaa atgtggtatg gctgattatg atcctctaga ctgcagcctc aggagatctg
      481 ggccctcact tgtacagctc atccatgccg tgggtgatgg cggcggcggt cacgaagccg
      541 aagtagatca tgtgatcgcg cttctcgttg gggtccttgg acagggcgct ctgggtggac
      601 aggtagtggt tatcgggcag cagcacaggg ccatcgccga tgggggtatt ctgctggtag
      661 tggtcggcca gctgcacgct gccatcctcg atgttgtggc ggatcttgaa gttcaccttg
      721 atgccattct tggccttgtc ggtcatgatg tacacattgt gggcgttgta gttgtactcc
      781 atcttattgc ccaggatgtt gccatcctcc ttgaaatcgg tgccggtcag ctcgatgcga
      841 ttcaccaggg tatcgccctc gaacttcacc tcggcgcgcg acttgtagtt gccgtcatcc
      901 tcgaagaaga tggtgcgctc ctggatgtag ccctcaggca tggcgctctt gaagaagtcg
      961 tgctgcttca tgtgatcggg gtagcgtgag aagcactgca cgccgtagct cagggtggtc
     1021 accagggtgg gccagggcac aggcagcttg ccggtggtgc agatgaactt cagggtcagc
     1081 ttgccgtagg tggcatcgcc ctcgccctcg ccgctcacgc tgaacttgtg gccattcaca
     1141 tcgccattca gctcgatcag gatgggcacg atgccggtga acagctcggc gcccttgctc
     1201 accatggtgg cgaattctcc aggcgatctg acggttcact aaacgagctc tgcttatata
     1261 ggcctcccac cgtacacgcc acctcgacat actcacgcgt tgacattgat tattgactag
     1321 ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg agttccgcgt
     1381 tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac
     1441 gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg
     1501 ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag
     1561 tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat
     1621 gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat
     1681 ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact cacggggatt
     1741 tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa atcaacggga
     1801 ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta ggcgtgtacg
     1861 gtgggaggtc tatataagca gagctctccc tatcagtgat agagatctcc ctatcagtga
     1921 tagagatcgt cgacgagctc gtttagtgaa ccgtcagatc gcctggagac gccatccacg
     1981 ctgttttgac ctccatagaa gacaccggga ccgatccagc ctccggactc tagcgtttaa
     2041 acttaagctc gcccttgcca ccatggtgag caagggcgag gaggataaca tggccatcat
     2101 caaggagttc atgcgcttca aggtgcacat ggagggctcc gtgaacggcc acgagttcga
     2161 gatcgagggc gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt
     2221 gaccaagggt ggccccctgc ccttcgcctg ggacatcctg tcccctcagt tcatgtacgg
     2281 ctccaaggcc tacgtgaagc accccgccga catccccgac tacttgaagc tgtccttccc
     2341 cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac
     2401 ccaggactcc tccctgcagg acggcgagtt catctacaag gtgaagctgc gcggcaccaa
     2461 cttcccctcc gacggccccg taatgcagaa gaagaccatg ggctgggagg cctcctccga
     2521 gagaatgtac cccgaggacg gcgccctgaa gggcgagatc aagcagaggc tgaagctgaa
     2581 ggacggcggc cactacgacg ctgaggtcaa gaccacctac aaggccaaga agcccgtgca
     2641 gctgcccggc gcctacaacg tcaacatcaa gttggacatc acctcccaca acgaggacta
     2701 caccatcgtg gaacagtacg aacgcgccga gggccgccac tccaccggcg gcatggacga
     2761 gctgtacaag taaggcggcc gcatcgataa gcttgtccac cggtacaagt ttgtacaaaa
     2821 aagctgaacg agaaacgtaa aatgatataa atatcaatat attaaattag attttgcata
     2881 aaaaacagac tacataatac tgtaaaacac aacatatcca gtcactatgg cggccgcatt
     2941 agggcacccc aggctttaca ctttatgctt ccggctcgta taatgtgtgg attttgagtt
     3001 aggatccgtc gggattttca ggagctaagg aagctaaaat ggagaaaaaa atcactggat
     3061 ataccaccgt tgatatatcc caatggcatc gtaaagaaca ttttgaggca tttcagtcag
     3121 ttgctcaatg tacctataac cagaccgttc agctggatat tacggccttt ttaaagaccg
     3181 taaagaaaaa taagcacaag ttttatccgg cctttattca cattcttgcc cgcctgatga
     3241 atgctcatcc ggaattccgt atggcaatga aagacggtga gctggtgata tgggatagtg
     3301 ttcacccttg ttacaccgtt ttccatgagc aaactgaaac gttttcatcg ctctggagtg
     3361 aataccacga cgatttccgg cagtttctac acatatattc gcaagatgtg gcgtgttacg
     3421 gtgaaaacct ggcctatttc cctaaagggt ttattgagaa tatgtttttc gtctcagcca
     3481 atccctgggt gagtttcacc agttttgatt taaacgtggc caatatggac aacttcttcg
     3541 cccccgtttt caccatgggc aaatattata cgcaaggcga caaggtgctg atgccgctgg
     3601 cgattcaggt tcatcatgcc gtctgtgatg gcttccatgt cggcagaatg cttaatgaat
     3661 tacaacagta ctgcgatgag tggcagggcg gggcgtaatc tagaggatcc ggcttactaa
     3721 aagccagata acagtatgcg tatttgcgcg ctgatttttg cggtataaga atatatactg
     3781 atatgtatac ccgaagtatg tcaaaaagag gtgtgctatg aagcagcgta ttacagtgac
     3841 agttgacagc gacagctatc agttgctcaa ggcatatatg atgtcaatat ctccggtctg
     3901 gtaagcacaa ccatgcagaa tgaagcccgt cgtctgcgtg ccgaacgctg gaaagcggaa
     3961 aatcaggaag ggatggctga ggtcgcccgg tttattgaaa tgaacggctc ttttgctgac
     4021 gagaacaggg actggtgaaa tgcagtttaa ggtttacacc tataaaagag agagccgtta
     4081 tcgtctgttt gtggatgtac agagtgatat tattgacacg cccgggcgac ggatggtgat
     4141 ccccctggcc agtgcacgtc tgctgtcaga taaagtcccc cgtgaacttt acccggtggt
     4201 gcatatcggg gatgaaagct ggcgcatgat gaccaccgat atggccagtg tgccggtctc
     4261 cgttatcggg gaagaagtgg ctgatctcag ccaccgcgaa aatgacatca aaaacgccat
     4321 taacctgatg ttctggggaa tataaatgtc aggctccctt atacacagcc agtctgcagg
     4381 tcgaccatag tgactggata tgttgtgttt tacagtatta tgtagtctgt tttttatgca
     4441 aaatctaatt taatatattg atatttatat cattttacgt ttctcgttca gctttcttgt
     4501 acaaagtggt ttaattcgaa aagcttgtcg acgatatctc cagaggatca agggcgagct
     4561 tggtaccgag ctcggatcca ctagtccagt gtggtggaat tctgcagata tccagcacag
     4621 tggcggccgc tcgagtctag agggcccgtt taaacccgct gatcagcctc gactgtgcct
     4681 tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt
     4741 gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg
     4801 tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagac
     4861 aatagcaggc atgctgggga tgcggtgggc tctatggctt ctgaggcgga aagaaccagc
     4921 tggggctcta gggggtatcc ccacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg
     4981 gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct
     5041 ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg
     5101 ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag
     5161 ggtgatggtt cacgtaccta gaagttccta ttccgaagtt cctattctct agaaagtata
     5221 ggaacttcct tggccaaaaa gcctgaactc accgcgacgt ctgtcgagaa gtttctgatc
     5281 gaaaagttcg acagcgtctc cgacctgatg cagctctcgg agggcgaaga atctcgtgct
     5341 ttcagcttcg atgtaggagg gcgtggatat gtcctgcggg taaatagctg cgccgatggt
     5401 ttctacaaag atcgttatgt ttatcggcac tttgcatcgg ccgcgctccc gattccggaa
     5461 gtgcttgaca ttggggaatt cagcgagagc ctgacctatt gcatctcccg ccgtgcacag
     5521 ggtgtcacgt tgcaagacct gcctgaaacc gaactgcccg ctgttctgca gccggtcgcg
     5581 gaggccatgg atgcgatcgc tgcggccgat cttagccaga cgagcgggtt cggcccattc
     5641 ggaccgcaag gaatcggtca atacactaca tggcgtgatt tcatatgcgc gattgctgat
     5701 ccccatgtgt atcactggca aactgtgatg gacgacaccg tcagtgcgtc cgtcgcgcag
     5761 gctctcgatg agctgatgct ttgggccgag gactgccccg aagtccggca cctcgtgcac
     5821 gcggatttcg gctccaacaa tgtcctgacg gacaatggcc gcataacagc ggtcattgac
     5881 tggagcgagg cgatgttcgg ggattcccaa tacgaggtcg ccaacatctt cttctggagg
     5941 ccgtggttgg cttgtatgga gcagcagacg cgctacttcg agcggaggca tccggagctt
     6001 gcaggatcgc cgcggctccg ggcgtatatg ctccgcattg gtcttgacca actctatcag
     6061 agcttggttg acggcaattt cgatgatgca gcttgggcgc agggtcgatg cgacgcaatc
     6121 gtccgatccg gagccgggac tgtcgggcgt acacaaatcg cccgcagaag cgcggccgtc
     6181 tggaccgatg gctgtgtaga agtactcgcc gatagtggaa accgacgccc cagcactcgt
     6241 ccgagggcaa aggaatagca cgtactacga gatttcgatt ccaccgccgc cttctatgaa
     6301 aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca gcgcggggat
     6361 ctcatgctgg agttcttcgc ccaccccaac ttgtttattg cagcttataa tggttacaaa
     6421 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
     6481 ggtttgtcca aactcatcaa tgtatcttat catgtctgta taccgtcgac ctctagctag
     6541 agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt
     6601 ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc
     6661 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc
     6721 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct
     6781 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
     6841 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     6901 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     6961 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     7021 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     7081 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     7141 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     7201 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     7261 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     7321 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     7381 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
     7441 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     7501 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     7561 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
     7621 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
     7681 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
     7741 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
     7801 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
     7861 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
     7921 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
     7981 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
     8041 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
     8101 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
     8161 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
     8221 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
     8281 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
     8341 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
     8401 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
     8461 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
     8521 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
     8581 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
     8641 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
     8701 gtgccacctg acgtc
//
