LOCUS       sequence-84092-e         855 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of 3xFLAG tagged TCAB1, N-terminal..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 855)
  AUTHORS   Venteicher AS, Abreu EB, Meng Z, McCann KE, Terns RM, Veenstra TD, 
            Terns MP, Artandi SE
  TITLE     A human telomerase holoenzyme protein required for Cajal body 
            localization and telomere synthesis.
  JOURNAL   Science. 2009 Jan 30;323(5914):644-8. doi: 10.1126/science.1165357.
  PUBMED    19179534
REFERENCE   2  (bases 1 to 855)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2009-01-30- 30"; volume: "323"; 
            issue: "5914"; pages: "644-8"
COMMENT     Sequence Label: pCDNA-3xFLAG-TCAB1
FEATURES             Location/Qualifiers
     source          1..855
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     48..67
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        48..66
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      145..154
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             166..231
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
ORIGIN
        1 agctctctgg ctactagaga acccactgct tactggctta tcgaaattaa tacgactcac
       61 tatagggaga cccaagctgg ctagttaagc ttggtaccga gctcggatcc actagtccag
      121 tgtggtggaa ttctgcagat aaacgccacc atggggtccc gggctgacta caaagaccat
      181 gacggtgatt ataaagatca tgacatcgac tacaaggatg acgatgacaa ggggcgcgcc
      241 aagactttgg agactcaacc gttagctccg gactgctgtc cttcagacca ggacccagct
      301 ccagcccatc cttctcccca cgcttccccg atgaataaaa atgcggactc tgaactgatg
      361 ccaccgcctc ccgaaagggg ggatccgccc cggttgtccc cagatcctgt ggctggctca
      421 gctgtgtccc aggagctacg ggagggggac ccagtttctc tctccactcc cctggaaaca
      481 gagtttggtt cccctagtga gttgagtcct cgaatcgagg agcaagaact ttctgaaaat
      541 acaagccttc ctgcagaaga agcaaacggg agcctttctg aagaagaagc gaacgggcca
      601 gagttggggt ctggaaaagc catggaagat acctctgggg aacccgctgc agaggacgag
      661 ggagacaccg cttggaacta cagcttctcc cagctgcctc gatttctcag tggttcctgg
      721 tcagagttca gcacccaacc tgagaacttc ttgaaaggct gtaagtgggc tcctgacggt
      781 tcctgcatct tgaccaatag tgctgataac atcttgcgaa tttataacct gcccccagag
      841 ctgtaccatg agggg
//
