LOCUS       sequence-84095-e         886 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of 3xFLAG tagged pontin, N-terminal..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 886)
  AUTHORS   Venteicher AS, Meng Z, Mason PJ, Veenstra TD, Artandi SE
  TITLE     Identification of ATPases pontin and reptin as telomerase components
            essential for holoenzyme assembly.
  JOURNAL   Cell. 2008 Mar 21;132(6):945-57. doi: 10.1016/j.cell.2008.01.019.
  PUBMED    18358808
REFERENCE   2  (bases 1 to 886)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2008.01"; journalName: "Cell"; date: "2008-03-21- 
            21"; volume: "132"; issue: "6"; pages: "945-57"
COMMENT     Sequence Label: pCDNA-3xFLAG-Pontin
FEATURES             Location/Qualifiers
     source          1..886
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     45..64
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        45..63
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      142..151
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             163..228
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
ORIGIN
        1 tctctggcta ctagagaacc cactgcttac tggcttatcg aaattaatac gactcactat
       61 agggagaccc aagctggcta gttaagcttg gtaccgagct cggatccact agtccagtgt
      121 ggtggaattc tgcagataaa cgccaccatg gggtcccggg ctgactacaa agaccatgac
      181 ggtgattata aagatcatga catcgactac aaggatgacg atgacaaggg gcgcgccaag
      241 attgaggagg tgaagagcac tacgaagacg cagcgcatcg cctcccacag ccacgtgaaa
      301 gggctggggc tggacgagag cggcttggcc aagcaggcgg cctcagggct tgtgggccag
      361 gagaacgcgc gagaggcatg tggcgtcata gtagaattaa tcaaaagcaa gaaaatggct
      421 ggaagagctg tcttgttggc aggacctcct ggaactggca agacagctct ggctctggct
      481 attgctcagg agctgggtag taaggtcccc ttctgcccaa tggtggggag tgaagtttac
      541 tcaactgaga tcaagaagac agaggtgctg atggagaact tccgcagggc cattgggctg
      601 cgaataaagg agaccaagga agtttatgaa ggtgaagtca cagagctaac tccgtgtgag
      661 acagagaatc ccatgggagg atatggcaaa accattagcc atgtgatcat aggactcaaa
      721 acagccaaag gaaccaaaca gttgaaactg gaccccagca tttttgaaag tttgcagaaa
      781 gagcgagtag aagctggaga tgtgatttac attgaagcca acagtggggc cgtgaagagg
      841 cagggcaggt gtgataccta tgccacagaa ttcgaccttg aagctg
//
