LOCUS       sequence-84096-e         806 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mamamlian expression of 3xHA tagged reptin, N-terminal..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 806)
  AUTHORS   Venteicher AS, Meng Z, Mason PJ, Veenstra TD, Artandi SE
  TITLE     Identification of ATPases pontin and reptin as telomerase components
            essential for holoenzyme assembly.
  JOURNAL   Cell. 2008 Mar 21;132(6):945-57. doi: 10.1016/j.cell.2008.01.019.
  PUBMED    18358808
REFERENCE   2  (bases 1 to 806)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2008.01"; journalName: "Cell"; date: "2008-03-21- 
            21"; volume: "132"; issue: "6"; pages: "945-57"
COMMENT     Sequence Label: pCDNA-3xHA-Reptin
FEATURES             Location/Qualifiers
     source          1..806
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     45..64
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        45..63
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      142..151
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             163..189
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             193..219
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             223..249
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 ctctctggct actgagaacc cactgcttac tggcttatcg aaattaatac gactcactat
       61 agggagaccc aagctggcta gttaagcttg gtaccgagct cggatccact agtccagtgt
      121 ggtggaattc tgcagataaa cgccaccatg gggcaacaag gatacccgta tgatgttccg
      181 gattacgctg gctacccata cgacgtccca gactacgctg gctacccata cgacgtccca
      241 gactacgctc aaggagtgcg gccgcggggg cgcgccgcaa ccgttacagc cacaaccaaa
      301 gtcccggaga tccgtgatgt aacaaggatt gagcgaatcg gtgcccactc ccacatccgg
      361 ggactggggc tggacgatgc cttggagcct cggcaggctt cgcaaggcat ggtgggtcag
      421 ctggcggcac ggcgggcggc tggcgtggtg ctggagatga tccgggaagg gaagattgcc
      481 ggtcgggcag tccttattgc tggccagccg ggcacgggga agacggccat cgccatgggc
      541 atggcgcagg ccctgggccc tgacacgcca ttcacagcca tcgccggcag tgaaatcttc
      601 tccctggaga tgagcaagac cgaggcgctg acgcaggcct tccggcggtc catcggcgtt
      661 cgcatcaagg aggagacgga gatcatcgaa ggggaggtgg tggagatcca gattgatcga
      721 ccagcaacag ggacgggctc caaggtgggc aaactgaccc tcaagaccac agagatggag
      781 accatctacg atttaggaac gaagat
//
