LOCUS       sequence-84105-e         845 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  AmpR + PCon:RBS (st7)::mCherry::T1 terminator + PLlacO-1::RBS (st7) 
            fimB::Asp terminator + ColE1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 845)
  AUTHORS   Hung M, Chang E, Hussein R, Frazier K, Shin JE, Sagawa S, Lim HN
  TITLE     Modulating the frequency and bias of stochastic switching to control
            phenotypic variation.
  JOURNAL   Nat Commun. 2014 Aug 4;5:4574. doi: 10.1038/ncomms5574.
  PUBMED    25087841
REFERENCE   2  (bases 1 to 845)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2014-08-4"; volume: "5:4574. doi"; pages: " 
            10.1038/ncomms5574"
COMMENT     Sequence Label: pHL 1917
FEATURES             Location/Qualifiers
     source          1..845
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        211..315
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(534..553)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 atctcgaact cgtggccgtt cacggagccc tccatgtgca ccttgaagcg catgaactcc
       61 ttgatgatgg ccatgttatc ctcctcgccc ttgctcacca ttttttttcc tccttacccg
      121 ggtgtgtgga atccattata accgccaagc ataaaaatgt caacaacgac ggtgctcgag
      181 acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
      241 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
      301 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
      361 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
      421 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
      481 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
      541 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat
      601 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
      661 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
      721 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
      781 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
      841 cgtga
//
