LOCUS       sequence-84106-e         869 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  AmpR + PCon:RBS (st7)::mCherry::T1 terminator + PLlacO-1::RBS (st7) 
            fimB::Asp terminator + ColE1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 869)
  AUTHORS   Hung M, Chang E, Hussein R, Frazier K, Shin JE, Sagawa S, Lim HN
  TITLE     Modulating the frequency and bias of stochastic switching to control
            phenotypic variation.
  JOURNAL   Nat Commun. 2014 Aug 4;5:4574. doi: 10.1038/ncomms5574.
  PUBMED    25087841
REFERENCE   2  (bases 1 to 869)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2014-08-4"; volume: "5:4574. doi"; pages: " 
            10.1038/ncomms5574"
COMMENT     Sequence Label: pHL 1917
FEATURES             Location/Qualifiers
     source          1..869
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     138..161
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     terminator      270..356
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     protein_bind    411..427
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 ccgaggaggc gccctgaagg gcgagatcaa gcagaggctg aagctgaagg acggcggcca
       61 ctacgacgcc gaggtcaaga ccacctacaa ggccaagaag cccgtgcagc ttcccggcgc
      121 ctacaacgtc aacatcaagc tggacatcac ctcccacaac gaggactaca ccatcgtgga
      181 acagtacgag cgcgccgagg gccgccactc aaccggcggc atggacgagc tgtacaagta
      241 agggcccatg gtacgcgtgc tagaggcatc aaataaaacg aaaggctcag tcgaaagact
      301 gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct cctgagtagg acaaatccgc
      361 cgccctagac ctaggcgcgg atccataaat gtgagcggat aacattgaca ttgtgagcgg
      421 ataacaagat actcccgggt aaggaggaaa aaaaatgaag aataaggctg ataacaaaaa
      481 aaggaacttc ctgacccata gtgaaatcga atcactcctt aaagcagcaa ataccgggcc
      541 tcatgcagca cgtaattatt gtctgacttt gctttgtttt attcatggtt tccgggcgag
      601 tgaaatttgt cgattgagga tttcggatat tgatcttaag gcaaagtgct gagagatccc
      661 ctcataattt ccccaaagcg taaccatgtg tgaataaatt ttgagctagt agggttgcag
      721 ccacgagtaa gtcttccctt gttattgtgt agccagaatg ccgcaaaact tccatgccta
      781 agcgaactgt tgagagtacg tttcgatttc tgactgtgtt agcctggaag tgcttgtccc
      841 aaccttgttt ctgagcatga acgcccgca
//
