LOCUS       sequence-84118-e         790 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pLlac0-1:RhyB-mCherry, pLtetO-1:RBS (st7) gfp
             .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 790)
  AUTHORS   Hussein R, Lim HN
  TITLE     Disruption of small RNA signaling caused by competition for Hfq.
  JOURNAL   Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 
            10.1073/pnas.1010082108. Epub 2010 Dec 28.
  PUBMED    21189298
REFERENCE   2  (bases 1 to 790)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1010082108"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2011-01-18- 18"; volume: "108"; issue: "3"; pages: "1110-5"
COMMENT     Sequence Label: pHL 1011
FEATURES             Location/Qualifiers
     source          1..790
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(57..74)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      227..313
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     protein_bind    365..381
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             523..534
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     primer_bind     complement(686..704)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 agctctacaa ataaaagcta gcttggctgt tttggcggat gagagaagat tttcagcctg
       61 atacagatta aatcagaacg cagaagcggt ctgataaaac agaatttgcc tggcggcagt
      121 agcgcggtgg tcccacctga ccccatgccg aactcagaag tgaaacgccg tagcgccgat
      181 ggtagtgtgg ggtctcccca tgcgagagta gggaactgcc aggcatcaaa taaaacgaaa
      241 ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcct
      301 gagtaggaca aatccgccgc cctagaccta ggggatccat aaatgtgagc ggataacatt
      361 gacattgtga gcggataaca agatactccc ggggcgatca ggaagaccct cgcggagaac
      421 ctgaaagcac gacattgctc acattgcttc cagtattact tagccagccg ggtgctggct
      481 ttttttttga tctttcgttc tcaatttatc cacaagctta ttaaagagga gaaacgtacg
      541 atggtgagca agggcgagga ggataacatg gccatcatca aggagttcat gcgcttcaag
      601 gtgcacatgg agggctccgt gaacggccac gagttcgaga tcgagggcga gggcgagggc
      661 cgcccctacg agggcaccca gaccgccaag ctgaaggtga ccaagggcgg ccccctgccc
      721 ttcgcctggg acatcctgtc ccctcagttc atgtacggct ccaaggccta cgtgaagcac
      781 cccgccgaca
//
