LOCUS       sequence-84121-e         795 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  PLtetO-1:hfqlead::mCherry, PLlacO-1:RBS(st7):csrA
             .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 795)
  TITLE     Lim Lab plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 795)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pHL1726
FEATURES             Location/Qualifiers
     source          1..795
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      271..357
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     protein_bind    412..428
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(749..766)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 cccgaggagg cgccctgaag ggcgagatca agcagaggct gaagctgaag gacggcggcc
       61 actacgacgc tgaggtcaag accacctaca aggccaagaa gcccgtgcag ctgcccggcg
      121 cctacaacgt caacatcaag ttggacatca cctcccacaa cgaggactac accatcgtgg
      181 aacagtacga acgcgccgag ggccgccact ccaccggcgg catggacgag ctgtacaagt
      241 aagggcccat ggtacgcgtg ctagaggcat caaataaaac gaaaggctca gtcgaaagac
      301 tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tcctgagtag gacaaatccg
      361 ccgccctaga cctaggcgcg gatccataaa tgtgagcgga taacattgac attgtgagcg
      421 gataacaaga tactcccggg taaggaggaa aaaaaatgct gattctgact cgtcgagttg
      481 gtgagaccct catgattggg gatgaggtca ccgtgacagt tttaggggta aagggcaacc
      541 aggtacgtat tggcgtaaat gccccgaagg aagtttctgt tcaccgtgaa gagatctacc
      601 agcgtatcca ggctgaaaaa tcccagcagt ccagttacta aggtacctga aagaagcttt
      661 aatcgtacag ggtagtacaa ataaaaaagg cacgtcagat gacgtgcctt ttttcttgtg
      721 agcagtgaat tcggatatat tccgcttcct cgctcactga ctcgctacgc tcggtcgttc
      781 gactgcggcg agcgg
//
