LOCUS       sequence-84122-e         232 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  PLtetO-1:hfqlead::mCherry, PLlacO-1:RBS(st7):csrA
             .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 232)
  TITLE     Lim Lab plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 232)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pHL1726
FEATURES             Location/Qualifiers
     source          1..232
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    148..164
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 aacttccttc ggggcattta cgccaatacg tacctggttg ccctttaccc ctaaaactgt
       61 cacggtgacc tcatccccaa tcatgagggt ctcaccaact cgacgagtca gaatcagcat
      121 tttttttcct ccttacccgg gagtatcttg ttatccgctc acaatgtcaa tgttatccgc
      181 tcacatttat ggatccgcgc ctaggtctag ggcggcggat ttgtcctact ca
//
