LOCUS       sequence-84123-e         914 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expression of TagRFP-tagged PSD-95.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 914)
  TITLE     Hell lab plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 914)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: PSD-95-pTagRFP
FEATURES             Location/Qualifiers
     source          1..914
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(128..841)
                     /codon_start=1
                     /product="monomeric derivative of red fluorescent protein 
                     from Entacmaea quadricolor (Merzlyak et al., 2007)"
                     /label=TagRFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFMYGSRTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTEMLYPADGGLEGRSD
                     MALKLVGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDHRLERIKEADKETYVEQHEVAV
                     ARYCDLPSKLGHKLN"
     regulatory      838..847
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 caagttaaca acaacaattg cattcatttt atgtttcagg ttcaggggga ggtgtgggag
       61 gttttttaaa gcaagtaaaa cctctacaaa tgtggtatgg ctgattatga tctagagtcg
      121 cggccgctca attaagtttg tgccccagtt tgctagggag gtcgcagtat ctggccacag
      181 ccacctcgtg ctgctcgacg taggtctctt tgtcggcctc cttgattctt tccagtctgt
      241 ggtccacata gtagacgccg ggcatcttga ggttcttagc gggtttcttg gatctgtatg
      301 tggtcttgaa gttgcagatc aggtggcccc cgcccacgag cttcagggcc atgtcgcttc
      361 tgccttccag gccgccgtca gcggggtaca gcatctcggt gttggcctcc cagccgagtg
      421 ttttcttctg catcacaggg ccgttggatg ggaagttcac ccctctgatc ttgacgttgt
      481 agatgaggca gccgtcctgg aggctggtgt cctgggtagc ggtcagcacg cccccgtctt
      541 cgtatgtggt gactctctcc catgtgaagc cctcagggaa ggactgctta aagaagtcgg
      601 ggatgccctg ggtgtggttg atgaaggttc tgctgccgta catgaagctg gtagccagga
      661 tgtcgaaggc gaaggggaga gggccgccct cgaccacctt gattctcatg gtctgggtgc
      721 cctcgtaggg cttgccttcg ccctcggatg tgcacttgaa gtggtggttg ttcacggtgc
      781 cctccatgta cagcttcatg tgcatgttct ccttaatcag ctcttcgccc ttagacacca
      841 tggtggcgac cggtggatcc cgggcccgcg gtaccgtcga ctgcagaatt ccgagtctct
      901 ctcgggctgg gaac
//
