LOCUS       sequence-84124-e         784 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  PLtetO-1:hfqlead::mCherry, PLlacO-1:RBS(st7):csrA
             .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 784)
  TITLE     Lim Lab plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 784)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pHL1726
FEATURES             Location/Qualifiers
     source          1..784
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    228..246
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    253..271
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     complement(292..310)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(543..562)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
ORIGIN
        1 tcgtggccgt tcacggagcc ctccatgtgc accttgaagc gcatgaactc cttgatgatg
       61 gccatgttat cctcctcgcc cttgctcagc atgcgttccc gacgcagtgc gttcaggaac
      121 ggatcttgta aagattgccc cttagccatt ctctcttttc cttatatgct tatttgtact
      181 ttgaaccttt cgattctgaa aaaattgcgc acgatacgtc gacagtatct ctatcactga
      241 tagggatgtc aatctctatc actgataggg actcgaggtg aagacgaaag ggcctcgtga
      301 tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcggaattgc
      361 cagctggggc gccctctggt aaggttggga agccctgcaa agtaaactgg atggctttct
      421 tgccgccaag gatctgatgg cgcaggggat caagatctga tcaagagaca ggatgaggat
      481 cgtttcgcat gattgaacaa ggtggattgc acgcaggttc tccggccgct tgggtggaga
      541 ggctattcgg ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc
      601 ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga
      661 atgaactgca ggacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg
      721 cagctgtgct cgacgttgtc actgaaagcg ggaagggact ggctgctatt gggcgaagtg
      781 ccgg
//
