LOCUS       sequence-84128-e         773 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pLtetO-1:RhyB, pCon:rpoS::mCherry, pLlacO-1:sodB::gfp
             .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 773)
  AUTHORS   Hussein R, Lim HN
  TITLE     Disruption of small RNA signaling caused by competition for Hfq.
  JOURNAL   Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 
            10.1073/pnas.1010082108. Epub 2010 Dec 28.
  PUBMED    21189298
REFERENCE   2  (bases 1 to 773)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1010082108"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2011-01-18- 18"; volume: "108"; issue: "3"; pages: "1110-5"
COMMENT     Sequence Label: pHL 1033
FEATURES             Location/Qualifiers
     source          1..773
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    587..605
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    612..630
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     terminator      complement(697..724)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ccctcgccct cgatctcgaa ctcgtggccg ttcacggagc cctccatgtg caccttgaag
       61 cgcatgaact ccttgatgat ggccatgtta tcctcctcgc ccttgctcac catttttttc
      121 ctccttagca tgctatcatg aactttcagc gtattctgac tcataaggtg gctcctaccc
      181 gtgatccctt gacggaacat tcaagcaaaa gcctggttcc gccgatttat cgctgcggca
      241 aataacgcag cgggtttacg gatttcccct tgtaacgaat ttcaaaatgc aagcgtgttg
      301 aactggttcc ggtgtcgacc tgtgtggaat ccattataac cgccaagctt aaaaatgtca
      361 acaacgacgg tgctcgagaa ttcactgctc acaagaaaaa aggcacgtca tctgacgtgc
      421 cttttttatt tgtactaccc tgtacgatta aagcttgtgg ataaattgag aacgaaagat
      481 caaaaaaaaa gccagcaccc ggctggctaa gtaatactgg aagcaatgtg agcaatgtcg
      541 tgctttcagg ttctccgcga gggtcttcct gatcgccccg ggagtatctc tatcactgat
      601 agggatgtca atctctatca ctgataggga ggatcctgag cggatacata tttgaatgta
      661 tttagaaaaa taaacaaaag agtttgtaga aacgcaaaaa ggccatccgt caggatggcc
      721 ttctgcttaa tttgatgcct ggcagtttat ggcgggcgtc ctgcccgcca ccc
//
