LOCUS       sequence-84129-e         886 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expression of TagRFP-tagged PSD-95_Y12E.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 886)
  TITLE     Hell lab plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 886)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: PSD-95_Y12E-pTagRFP
FEATURES             Location/Qualifiers
     source          1..886
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(130..843)
                     /codon_start=1
                     /product="monomeric derivative of red fluorescent protein 
                     from Entacmaea quadricolor (Merzlyak et al., 2007)"
                     /label=TagRFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFMYGSRTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTEMLYPADGGLEGRSD
                     MALKLVGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDHRLERIKEADKETYVEQHEVAV
                     ARYCDLPSKLGHKLN"
     regulatory      840..849
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 aacaagttaa caacaacaat tgcattcatt ttatgtttca ggttcagggg gaggtgtggg
       61 aggtttttta aagcaagtaa aacctctaca aatgtggtat ggctgattat gatctagagt
      121 cgcggccgct caattaagtt tgtgccccag tttgctaggg aggtcgcagt atctggccac
      181 agccacctcg tgctgctcga cgtaggtctc tttgtcggcc tccttgattc tttccagtct
      241 gtggtccaca tagtagacgc cgggcatctt gaggttctta gcgggtttct tggatctgta
      301 tgtggtcttg aagttgcaga tcaggtggcc cccgcccacg agcttcaggg ccatgtcgct
      361 tctgccttcc aggccgccgt cagcggggta cagcatctcg gtgttggcct cccagccgag
      421 tgttttcttc tgcatcacag ggccgttgga tgggaagttc acccctctga tcttgacgtt
      481 gtagatgagg cagccgtcct ggaggctggt gtcctgggta gcggtcagca cgcccccgtc
      541 ttcgtatgtg gtgactctct cccatgtgaa gccctcaggg aaggactgct taaagaagtc
      601 ggggatgccc tgggtgtggt tgatgaaggt tctgctgccg tacatgaagc tggtagccag
      661 gatgtcgaag gcgaagggga gagggccgcc ctcgaccacc ttgattctca tggtctgggt
      721 gccctcgtag ggcttgcctt cgccctcgga tgtgcacttg aagtggtggt tgttcacggt
      781 gccctccatg tacagcttca tgtgcatgtt ctccttaatc agctcttcgc ccttagacac
      841 catggtggcg accggtggat cccgggcccg cggtaccgtc gactgc
//
