LOCUS       sequence-84134-e         841 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pLtetO-1:OxyS-mCherry, pLlacO-1:ompC::gfp
             .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 841)
  AUTHORS   Hussein R, Lim HN
  TITLE     Disruption of small RNA signaling caused by competition for Hfq.
  JOURNAL   Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 
            10.1073/pnas.1010082108. Epub 2010 Dec 28.
  PUBMED    21189298
REFERENCE   2  (bases 1 to 841)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1010082108"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2011-01-18- 18"; volume: "108"; issue: "3"; pages: "1110-5"
COMMENT     Sequence Label: pHL 802
FEATURES             Location/Qualifiers
     source          1..841
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             119..130
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     protein_bind    299..317
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    324..342
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     terminator      complement(409..436)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(528..614)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     766..783
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
ORIGIN
        1 cctcgccctc gatctcgaac tcgtggccgt tcacggagcc ctccatgtgc accttgaagc
       61 gcatgaactc cttgatgatg gccatgttat cctcctcgcc cttgctcacc atcgtacgtt
      121 tctcctcttt aataagcttt gagcctggct tatcgccggg cttttttatg gcaaaaaaaa
      181 gcggatcctg gagatccgca aaagttcacg ttggctttag ttattcgagt tgagaaactc
      241 tcgaaacggg cagtgacttc aagggttaaa agaggtgccg ctccgtttcc cgggagtatc
      301 tctatcactg atagggatgt caatctctat cactgatagg gaggatcctg agcggataca
      361 tatttgaatg tatttagaaa aataaacaaa agagtttgta gaaacgcaaa aaggccatcc
      421 gtcaggatgg ccttctgctt aatttgatgc ctggcagttt atggcgggcg tcctgcccgc
      481 caccctccgg gccgttgctt cgcaacgttc aaatccgctc ccggcggatt tgtcctactc
      541 aggagagcgt tcaccgacaa acaacagata aaacgaaagg cccagtcttt cgactgagcc
      601 tttcgtttta tttgatgcct ggcagttccc tactctcgca tggggagacc cacactacca
      661 tcggcgctac ggcgtttcac ttctgagttc ggcatggggt caggtgggac caccgcgcta
      721 ctgccgccag gcaaattctg ttttatcaga ccgcttctgc gttctgattt aatctgtatc
      781 aggctgaaaa tcttctctca tccgccaaaa cagccaagct agcttttatt tgtagagctc
      841 a
//
