LOCUS       sequence-84136-e         807 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pLtetO-1:RhyB, pCon:OxyS, pLlacO-1:sodB::gfp
             .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 807)
  AUTHORS   Hussein R, Lim HN
  TITLE     Disruption of small RNA signaling caused by competition for Hfq.
  JOURNAL   Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 
            10.1073/pnas.1010082108. Epub 2010 Dec 28.
  PUBMED    21189298
REFERENCE   2  (bases 1 to 807)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1010082108"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2011-01-18- 18"; volume: "108"; issue: "3"; pages: "1110-5"
COMMENT     Sequence Label: pHL 1022
FEATURES             Location/Qualifiers
     source          1..807
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    150..168
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    175..193
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     terminator      complement(260..287)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(379..465)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     618..635
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     primer_bind     complement(721..741)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 ccctgtacga ttaaagcttg tggataaatt gagaacgaaa gatcaaaaaa aaagccagca
       61 cccggctggc taagtaatac tggaagcaat gtgagcaatg tcgtgctttc aggttctccg
      121 cgagggtctt cctgatcgcc ccgggagtat ctctatcact gatagggatg tcaatctcta
      181 tcactgatag ggaggatcct gagcggatac atatttgaat gtatttagaa aaataaacaa
      241 aagagtttgt agaaacgcaa aaaggccatc cgtcaggatg gccttctgct taatttgatg
      301 cctggcagtt tatggcgggc gtcctgcccg ccaccctccg ggccgttgct tcgcaacgtt
      361 caaatccgct cccggcggat ttgtcctact caggagagcg ttcaccgaca aacaacagat
      421 aaaacgaaag gcccagtctt tcgactgagc ctttcgtttt atttgatgcc tggcagttcc
      481 ctactctcgc atggggagac cccacactac catcggcgct acggcgtttc acttctgagt
      541 tcggcatggg gtcaggtggg accaccgcgc tactgccgcc aggcaaattc tgttttatca
      601 gaccgcttct gcgttctgat ttaatctgta tcaggctgaa aatcttctct catccgccaa
      661 aacagccaag ctagctttta tttgtagagc tcatccatgc catgtgtaat cccagcagct
      721 gttacaaact caagaaggac catgtggtct ctcttttcgt tgggatcttt cgaaagggca
      781 gattgtgtgg acaggtaatg gttgtct
//
