LOCUS       sequence-84142-e         824 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pLtetO-1:MicC, pLtetO-1:sodB::mCherry, pLlacO-1:ompC::gfp
             .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 824)
  AUTHORS   Hussein R, Lim HN
  TITLE     Disruption of small RNA signaling caused by competition for Hfq.
  JOURNAL   Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 
            10.1073/pnas.1010082108. Epub 2010 Dec 28.
  PUBMED    21189298
REFERENCE   2  (bases 1 to 824)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1010082108"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2011-01-18- 18"; volume: "108"; issue: "3"; pages: "1110-5"
COMMENT     Sequence Label: pHL 762
FEATURES             Location/Qualifiers
     source          1..824
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    345..363
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    370..388
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    611..629
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    636..654
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     terminator      complement(721..748)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
ORIGIN
        1 cgccctcgcc ctcgatctcg aactcgtggc cgttcacgga gccctccatg tgcaccttga
       61 agcgcatgaa ctccttgatg atggccatgt tatcctcctc gcccttgctc accatttttt
      121 ttcctcctta gcatgctcgc ggtaccttta atcaggttgt tcaggttagt gacataagtc
      181 tgatggtgct tgccgtagtg atactcgatg gtttccgcag aaatgtgcgg tgccagagca
      241 tctttagcat atggtagtgc aggtaattcg aatgacattg ctactctcct ttattattaa
      301 tttgcatacg tacaatagcc ttattgtgcg tatggtcgac agtatctcta tcactgatag
      361 ggatgtcaat ctctatcact gatagggaga attcactgct cacaagaaaa aaggcacgtc
      421 atctgacgtg ccttttttat ttgtactacc ctgtacgatt aaagcttctg gataaggatt
      481 atccaattct aaaaaaaaag cccggacgac tgttcgggct tgtcttttta tatgttggaa
      541 aatcagtggc aatgcaatgg cccaacagaa aataaaatct gtgacaataa aggcatataa
      601 cccgggagta tctctatcac tgatagggat gtcaatctct atcactgata gggaggatcc
      661 tgagcggata catatttgaa tgtatttaga aaaataaaca aaagagtttg tagaaacgca
      721 aaaaggccat ccgtcaggat ggccttctgc ttaatttgat gcctggcagt ttatggcggg
      781 cgtcctgccc gccaccctcc gggccgttgc ttcgcaacgt tcaa
//
