LOCUS       sequence-84150-e         891 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  shRNA against alpha-actinin-1 with eGFP transfection marker .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 891)
  AUTHORS   Schnizler MK, Schnizler K, Zha XM, Hall DD, Wemmie JA, Hell JW, 
            Welsh MJ
  TITLE     The cytoskeletal protein alpha-actinin regulates acid-sensing ion 
            channel 1a through a C-terminal interaction.
  JOURNAL   J Biol Chem. 2009 Jan 30;284(5):2697-705. doi: 
            10.1074/jbc.M805110200. Epub 2008 Nov 21.
  PUBMED    19028690
REFERENCE   2  (bases 1 to 891)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M805110200"; journalName: "J Biol Chem"; date: 
            "2009-01-30- 30"; volume: "284"; issue: "5"; pages: "2697-705"
COMMENT     Sequence Label: pSIL-eGFP-shACTN1
FEATURES             Location/Qualifiers
     source          1..891
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     39..55
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(133..446)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for mouse U6 snRNA (Das 
                     et al., 1988)"
     primer_bind     complement(194..214)
                     /label=mU6-F
                     /note="Mouse U6 promoter, forward primer"
     primer_bind     complement(443..459)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(516..537)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     865..884
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 gggaacaaaa gctggagctc caccgcggtg gcggccgctc tagaactagt ggatcccccg
       61 ggctgcagga attaaaaaag atctccaacg tcaacaagtc tcttgaactt gttgacgttg
      121 gagatcggcc caaacaaggc ttttctccaa gggatattta tagtctcaaa acacacaatt
      181 actttacagt tagggtgagt ttccttttgt gctgtttttt aaaataataa tttagtattt
      241 gtatctctta tagaaatcca agcctatcat gtaaaatgta gctagtatta aaaagaacag
      301 attatctgtc ttttatcgca cattaagcct ctatagttac taggaaatat tatatgcaaa
      361 ttaaccgggg caggggagta gccgagcttc tcccacaagt ctgtgcgagg gggccggcgc
      421 gggcctagag atggcggcgt cggatccact agttctagag cgggtaccta gtacttgtac
      481 agctcgtcca tgccgagagt gatcccggcg gcggtcacga actccagcag gaccatgtga
      541 tcgcgcttct cgttggggtc tttgctcagg gcggactggg tgctcaggta gtggttgtcg
      601 ggcagcagca cggggccgtc gccgatgggg gtgttctgct ggtagtggtc ggcgagctgc
      661 acgctgccgt cctcgatgtt gtggcggatc ttgaagttca ccttgatgcc gttcttctgc
      721 ttgtcggcca tgatatagac gttgtggctg ttgtagttgt actccagctt gtgccccagg
      781 atgttgccgt cctccttgaa gtcgatgccc ttcagctcga tgcggttcac cagggtgtcg
      841 ccctcgaact tcacctcggc gcgggtcttg tagttgccgt cgtccttgaa g
//
