LOCUS       sequence-84170-e        1917 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Actin, Excitation: 487, Emission: 509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1917)
  TITLE     Davidson mEmerald
REFERENCE   2  (bases 1 to 1917)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEmerald-Actin-7
FEATURES             Location/Qualifiers
     source          1..1917
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      27..36
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(33..35,36..38,39..749)
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(78..99)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(339..358)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     686..707
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 cgtcagatcc gctagcgcta ccggtcgcca ccatggtgag caagggcgag gagctgttca
       61 ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg
      121 tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca
      181 ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccttgacc tacggcgtgc
      241 agtgcttcgc ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc
      301 ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc
      361 gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg
      421 acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca
      481 aggtctatat caccgccgac aagcagaaga acggcatcaa ggtgaacttc aagacccgcc
      541 acaacatcga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg
      601 gcgacggccc cgtgctgctg cccgacaacc actacctgag cacccagtcc aagctgagca
      661 aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga
      721 tcactctcgg catggacgag ctgtacaagt ccggactcag atctcgagct atggatgatg
      781 atatcgccgc gctcgtcgtc gacaacggct ccggcatgtg caaggccggc ttcgcgggcg
      841 acgatgcccc ccgggccgtc ttcccctcca tcgtggggcg ccccaggcac cagggcgtga
      901 tggtgggcat gggtcagaag gattcctatg tgggcgacga ggcccagagc aagagaggca
      961 tcctcaccct gaagtacccc atcgagcacg gcatcgtcac caactgggac gacatggaga
     1021 aaatctggca ccacaccttc tacaatgagc tgcgtgtggc tcccgaggag caccccgtgc
     1081 tgctgaccga ggcccccctg aaccccaagg ccaaccgcga gaagatgacc cagatcatgt
     1141 ttgagacctt caacacccca gccatgtacg ttgctatcca ggctgtgcta tccctgtacg
     1201 cctctggccg taccactggc atcgtgatgg actccggtga cggggtcacc cacactgtgc
     1261 ccatctacga ggggtatgcc ctcccccatg ccatcctgcg tctggacctg gctggccggg
     1321 acctgactga ctacctcatg aagatcctca ccgagcgcgg ctacagcttc accaccacgg
     1381 ccgagcggga aatcgtgcgt gacattaagg agaagctgtg ctacgtcgcc ctggacttcg
     1441 agcaagagat ggccacggct gcttccagct cctccctgga gaagagctac gagctgcctg
     1501 acggccaggt catcaccatt ggcaatgagc ggttccgctg ccctgaggca ctcttccagc
     1561 cttccttcct gggcatggag tcctgtggca tccacgaaac taccttcaac tccatcatga
     1621 agtgtgacgt ggacatccgc aaagacctgt acgccaacac agtgctgtct ggcggcacca
     1681 ccatgtaccc tggcattgcc gacaggatgc agaaggagat cactgccctg gcacccagca
     1741 caatgaagat caagatcatt gctcctcctg agcgcaagta ctccgtgtgg atcggcggct
     1801 ccatcctggc ctcgctgtcc accttccagc agatgtggat cagcaagcag gagtatgacg
     1861 agtccggccc ctccatcgtc caccgcaaat gcttctaggg atccaccgga tctagat
//
