LOCUS       sequence-84172-e        1932 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Actin, Excitation: 487, Emission: 509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1932)
  TITLE     Davidson mEmerald
REFERENCE   2  (bases 1 to 1932)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEmerald-Actin-N-10
FEATURES             Location/Qualifiers
     source          1..1932
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      15..24
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      1170..1179
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1176..1178,1179..1181,1182..1895)
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1221..1242)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1482..1501)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1829..1850
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 tccggctagc ggacgccacc atggatgatg atatcgccgc gctcgtcgtc gacaacggct
       61 ccggcatgtg caaggccggc ttcgcgggcg acgatgcccc ccgggccgtc ttcccctcca
      121 tcgtggggcg ccccaggcac cagggcgtga tggtgggcat gggtcagaag gattcctatg
      181 tgggcgacga ggcccagagc aagagaggca tcctcaccct gaagtacccc atcgagcacg
      241 gcatcgtcac caactgggac gacatggaga aaatctggca ccacaccttc tacaatgagc
      301 tgcgtgtggc tcccgaggag caccccgtgc tgctgaccga ggcccccctg aaccccaagg
      361 ccaaccgcga gaagatgacc cagatcatgt ttgagacctt caacacccca gccatgtacg
      421 ttgctatcca ggctgtgcta tccctgtacg cctctggccg taccactggc atcgtgatgg
      481 actccggtga cggggtcacc cacactgtgc ccatctacga ggggtatgcc ctcccccatg
      541 ccatcctgcg tctggacctg gctggccggg acctgactga ctacctcatg aagatcctca
      601 ccgagcgcgg ctacagcttc accaccacgg ccgagcggga aatcgtgcgt gacattaagg
      661 agaagctgtg ctacgtcgcc ctggacttcg agcaagagat ggccacggct gcttccagct
      721 cctccctgga gaagagctac gagctgcctg acggccaggt catcaccatt ggcaatgagc
      781 ggttccgctg ccctgaggca ctcttccagc cttccttcct gggcatggag tcctgtggca
      841 tccacgaaac taccttcaac tccatcatga agtgtgacgt ggacatccgc aaagacctgt
      901 acgccaacac agtgctgtct ggcggcacca ccatgtaccc tggcattgcc gacaggatgc
      961 agaaggagat cactgccctg gcacccagca caatgaagat caagatcatt gctcctcctg
     1021 agcgcaagta ctccgtgtgg atcggcggct ccatcctggc ctcgctgtcc accttccagc
     1081 agatgtggat cagcaagcag gagtatgacg agtccggccc ctccatcgtc caccgcaaat
     1141 gcttcagctc cggaggggat ccaccggtcg ccaccatggt gagcaagggc gaggagctgt
     1201 tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca
     1261 gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct
     1321 gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccttg acctacggcg
     1381 tgcagtgctt cgcccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca
     1441 tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga
     1501 cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca
     1561 tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc
     1621 acaaggtcta tatcaccgcc gacaagcaga agaacggcat caaggtgaac ttcaagaccc
     1681 gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca
     1741 tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcacccag tccaagctga
     1801 gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg
     1861 ggatcactct cggcatggac gagctgtaca agtaagcggc cgcgactcta gatcataatc
     1921 agccatacca cc
//
