LOCUS       sequence-84173-e        2365 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Membrane Receptor, Excitation: 487, Emission: 509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2365)
  TITLE     Davidson mEmerald
REFERENCE   2  (bases 1 to 2365)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEmerald-ALK1-C-18
FEATURES             Location/Qualifiers
     source          1..2365
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      32..41
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(38..40,41..43,44..754)
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(83..104)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(344..363)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     691..712
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 agtgacgtca gatccgctag cgctaccggt cgccaccatg gtgagcaagg gcgaggagct
       61 gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt
      121 cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat
      181 ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccacct tgacctacgg
      241 cgtgcagtgc ttcgcccgct accccgacca catgaagcag cacgacttct tcaagtccgc
      301 catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa
      361 gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg
      421 catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag
      481 ccacaaggtc tatatcaccg ccgacaagca gaagaacggc atcaaggtga acttcaagac
      541 ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc
      601 catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccaagct
      661 gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc
      721 cgggatcact ctcggcatgg acgagctgta caagtccgga ctcagatctc gagctcaagc
      781 ttcgaattca gggtctggaa gtggtagcac cttgggctcc cccaggaaag gccttctgat
      841 gctgctgatg gccttggtga cccagggaga ccctgtgaag ccgtctcggg gcccgctggt
      901 gacctgcacg tgtgagagcc cacattgcaa ggggcctacc tgccgggggg cctggtgcac
      961 agtagtgctg gtgcgggagg aggggaggca cccccaggaa catcggggct gcgggaactt
     1021 gcacagggag ctctgcaggg ggcgccccac cgagttcgtc aaccactact gctgcgacag
     1081 ccacctctgc aaccacaacg tgtccctggt gctggaggcc acccaacctc cttcggagca
     1141 gccgggaaca gatggccagc tggccctgat cctgggcccc gtgctggcct tgctggccct
     1201 ggtggccctg ggtgtcctgg gcctgtggca tgtccgacgg aggcaggaga agcagcgtgg
     1261 cctgcacagc gagctgggag agtccagtct catcctgaaa gcatctgagc agggcgacag
     1321 catgttgggg gacctcctgg acagtgactg caccacaggg agtggctcag ggctcccctt
     1381 cctggtgcag aggacagtgg cacggcaggt tgccttggtg gagtgtgtgg gaaaaggccg
     1441 ctatggcgaa gtgtggcggg gcttgtggca cggtgagagt gtggccgtca agatcttctc
     1501 ctcgagggat gaacagtcct ggttccggga gactgagatc tataacacag tgttgctcag
     1561 acacgacaac atcctaggct tcatcgcctc agacatgacc tcccgcaact cgagcacgca
     1621 gctgtggctc atcacgcact accacgagca cggctccctc tacgactttc tgcagagaca
     1681 gacgctggag ccccatctgg ctctgaggct agctgtgtcc gcggcatgcg gcctggcgca
     1741 cctgcacgtg gagatcttcg gtacacaggg caaaccagcc attgcccacc gcgacttcaa
     1801 gagccgcaat gtgctggtca agagcaacct gcagtgttgc atcgccgacc tgggcctggc
     1861 tgtgatgcac tcacagggca gcgattacct ggacatcggc aacaacccga gagtgggcac
     1921 caagcggtac atggcacccg aggtgctgga cgagcagatc cgcacggact gctttgagtc
     1981 ctacaagtgg actgacatct gggcctttgg cctggtgctg tgggagattg cccgccggac
     2041 catcgtgaat ggcatcgtgg aggactatag accacccttc tatgatgtgg tgcccaatga
     2101 ccccagcttt gaggacatga agaaggtggt gtgtgtggat cagcagaccc ccaccatccc
     2161 taaccggctg gctgcagacc cggtcctctc aggcctagct cagatgatgc gggagtgctg
     2221 gtacccaaac ccctctgccc gactcaccgc gctgcggatc aagaagacac tacaaaaaat
     2281 tagcaacagt ccagagaagc ctaaagtgat tcaataggga tccaccggat ctagataact
     2341 gatcataatc agccatacca cattg
//
