LOCUS       sequence-84176-e        3545 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Tight Junctions, Excitation: 487, Emission: 509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3545)
  TITLE     Davidson mEmerald
REFERENCE   2  (bases 1 to 3545)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEmerald-Alpha1-Catenin-N-18
FEATURES             Location/Qualifiers
     source          1..3545
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      2788..2797
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(2794..2796,2797..2799,2800..3513)
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2839..2860)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3100..3119)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3447..3468
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gtcagatccg ctagcaccgc catgactgcc gtccacgcag gcaacataaa cttcaaatgg
       61 gaccccaaaa gtctggagat taggactctg gctgttgaaa gactgttgga gcctcttgtt
      121 acacaggtta caaccctggt aaacaccaat agtaaagggc cgtctaataa aaagagaggc
      181 cgttctaaga aggcccatgt tttggctgca tctgttgaac aagcaactga gaatttcttg
      241 gaaaaggggg ataaaattgc aaaagagagc cagtttctca aggaggagct tgtggttgct
      301 gtagaagatg ttcgaaaaca aggagatttg atgaagagcg ctgctgggga gtttgcagat
      361 gatccatgct cttctgtgaa gcgaggcaac atggtccggg cagctcgagc tttgctctct
      421 gccgtcaccc ggctgctgat tctggctgac atggcagatg tctacaaatt acttgttcag
      481 ctgaaagttg tggaagatgg tatattgaaa ctgaggaatg ctggcaatga acaagactta
      541 gggatacagt acaaagctct gaaaccagaa gtagataagc tgaacatcat ggcagcaaaa
      601 agacaacagg aactaaaaga tgtgggcaat cgtgatcaga tggctgcagc tagaggaatc
      661 ctgcagaaaa atgttccaat cctctatact gcatcccagg catgtctaca acaccctgat
      721 gttgcagcct ataaggccaa ccgggacttg atatacaagc agctgcagca ggccgtcaca
      781 ggaatttcta atgcagccca ggctactgca tcagatgatg ctgcccagca ccagggtggc
      841 agtggcggag agctggcata cgctctcaac aactttgata aacaaatcat tgtggacccc
      901 ttgagcttca gcgaggagcg ctttaggccg tccctggagg agcgcctgga aagcattatt
      961 agtggggctg ccctgatggc tgactcatcc tgcacacgtg atgaccgtcg ggagcgcatt
     1021 gtggcagagt gtaatgctgt ccgccaggct ctacaggacc tgctctcaga atacatgggc
     1081 aacgctggac gtaaagaaag aagtgatgca ctcaactctg caatagataa aatgaccaag
     1141 aagaccaggg acttgcgtag acagcttcgc aaagctgtca tggaccacgt atcagattct
     1201 ttcctggaga ccaatgtccc tctattagta ctgattgaag ctgcaaagaa tggaaatgag
     1261 aaagaagtta aggaatatgc ccaagttttt cgtgaacatg ccaacaaact gattgaggtt
     1321 gccaacctgg cctgttcaat ctccaacaat gaagaaggcg tgaagcttgt ccgaatgtct
     1381 gcaagccagt tagaagcgct gtgtcctcag gttatcaatg ctgcattggc gttggcagca
     1441 aagccgcaga gtaaactggc ccaagagaac atggatcttt ttaaagagca atgggaaaag
     1501 caagtccgtg ttctcacaga tgctgttgat gacattactt ccatcgatga cttcttggct
     1561 gtctcagaga accacatttt agaagatgtg aacaagtgtg ttattgctct ccaagagaaa
     1621 gacgtggatg ggctggatcg cactgccggt gccatccgag gccgggcagc ccgagtcatt
     1681 catgtagtca cctcagagat ggacaactac gagccaggag tttacacaga gaaggttctg
     1741 gaagccacca agctcctctc caacacagtc atgccgcgct tcactgagca ggtggaagca
     1801 gctgtggaag ccctcagctc ggacccggcc cagcccatgg atgagaatga gttcatcgac
     1861 gcctcccgtc tggtgtacga cggcatccgg gacatccgga aagcagtgct gatgatcagg
     1921 acccccgagg agttggacga ctctgacttc gagactgaag actttgatgt cagaagcagg
     1981 accagtgtcc agacagaaga tgaccagctg atagctggcc agagcgcccg ggcgatcatg
     2041 gctcagcttc ctcaggagca aaaagcaaag attgcagaac aggtggccag tttccaggaa
     2101 gagaagagca agctggatgc tgaagtgtcc aagtgggatg acagtggcaa tgacatcatt
     2161 gtgctggcca agcagatgtg catgatcatg atggagatga ccgacttcac ccgaggcaaa
     2221 ggtccactca aaaacacctc tgacgtgatc agtgctgcca agaaaattgc ggaggcggga
     2281 tccaggatgg acaagctcgg ccggaccatc gcagaccatt gcccagactc ggcatgcaag
     2341 caggacctgc tggcctacct tcagcgcatc gctctctact gccaccagct caacatctgc
     2401 agcaaagtca aggctgaagt acagaacctt ggtggagagc tggtcgtctc tggggtggac
     2461 agcgccatgt ccctgatcca ggcggccaag aacttgatga atgctgttgt gcagacagtg
     2521 aaggcgtcct acgtggcttc caccaaatac cagaagtcac aggggatggc ttccttgaac
     2581 cttcctgctg tgtcatggaa gatgaaggcc cctgagaaga agccgttggt gaagagagag
     2641 aagcaggatg agacgcagac caagattaaa cgagcttctc agaagaaaca cgtgaaccca
     2701 gtgcaggccc tgagcgagtt caaagccatg gacagcatca gtcgcggagg cagtggtggg
     2761 gtaccgcggg cccgggatcc accggtcgcc accatggtga gcaagggcga ggagctgttc
     2821 accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc
     2881 gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc
     2941 accaccggca agctgcccgt gccctggccc accctcgtga ccaccttgac ctacggcgtg
     3001 cagtgcttcg cccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg
     3061 cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc
     3121 cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc
     3181 gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta caacagccac
     3241 aaggtctata tcaccgccga caagcagaag aacggcatca aggtgaactt caagacccgc
     3301 cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc
     3361 ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc caagctgagc
     3421 aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg
     3481 atcactctcg gcatggacga gctgtacaag taagcggccg cgactctaga tcataatcag
     3541 ccata
//
