LOCUS       sequence-84178-e        3997 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Focal Adhesions, Excitation: 487, Emission: 509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3997)
  TITLE     Davidson mEmerald
REFERENCE   2  (bases 1 to 3997)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEmerald-Alpha-V-Integrin-25
FEATURES             Location/Qualifiers
     source          1..3997
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    3173..3225
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      3235..3244
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(3241..3243,3244..3246,3247..3960)
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3286..3307)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3547..3566)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3894..3915
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 ctagtcgacg gtacctcggc gatggctttt ccgccgcggc gacggctgcg cctcggtccc
       61 cgcggcctcc cgcttcttct ctcgggactc ctgctacctc tgtgccgcgc cttcaaccta
      121 gacgtggaca gtcctgccga gtactctggc cccgagggaa gttacttcgg cttcgccgtg
      181 gatttcttcg tgcccagcgc gtcttcccgg atgtttcttc tcgtgggagc tcccaaagca
      241 aacaccaccc agcctgggat tgtggaagga gggcaggtcc tcaaatgtga ctggtcttct
      301 acccgccggt gccagccaat tgaatttgat gcaacaggca atagagatta tgccaaggat
      361 gatccattgg aatttaagtc ccatcagtgg tttggagcat ctgtgaggtc gaaacaggat
      421 aaaattttgg cctgtgcccc attgtaccat tggagaactg agatgaaaca ggagcgagag
      481 cctgttggaa catgctttct tcaagatgga acaaagactg ttgagtatgc tccatgtaga
      541 tcacaagata ttgatgctga tggacaggga ttttgtcaag gaggattcag cattgatttt
      601 actaaagctg acagagtact tcttggtggt cctggtagct tttattggca aggtcagctt
      661 atttcggatc aagtggcaga aatcgtatct aaatacgacc ccaatgttta cagcatcaag
      721 tataataacc aattagcaac tcggactgca caagctattt ttgatgacag ctatttgggt
      781 tattctgtgg ctgtcggaga tttcaatggt gatggcatag atgactttgt ttcaggagtt
      841 ccaagagcag caaggacttt gggaatggtt tatatttatg atgggaagaa catgtcctcc
      901 ttatacaatt ttactggcga gcagatggct gcatatttcg gattttctgt agctgccact
      961 gacattaatg gagatgatta tgcagatgtg tttattggag cacctctctt catggatcgt
     1021 ggctctgatg gcaaactcca agaggtgggg caggtctcag tgtctctaca gagagcttca
     1081 ggagacttcc agacgacaaa gctgaatgga tttgaggtct ttgcacggtt tggcagtgcc
     1141 atagctcctt tgggagatct ggaccaggat ggtttcaatg atattgcaat tgctgctcca
     1201 tatgggggtg aagataaaaa aggaattgtt tatatcttca atggaagatc aacaggcttg
     1261 aacgcagtcc catctcaaat ccttgaaggg cagtgggctg ctcgaagcat gccaccaagc
     1321 tttggctatt caatgaaagg agccacagat atagacaaaa atggatatcc agacttaatt
     1381 gtaggagctt ttggtgtaga tcgagctatc ttatacaggg ccagaccagt tatcactgta
     1441 aatgctggtc ttgaagtgta ccctagcatt ttaaatcaag acaataaaac ctgctcactg
     1501 cctggaacag ctctcaaagt ttcctgtttt aatgttaggt tctgcttaaa ggcagatggc
     1561 aaaggagtac ttcccaggaa acttaatttc caggtggaac ttcttttgga taaactcaag
     1621 caaaagggag caattcgacg agcactgttt ctctacagca ggtccccaag tcactccaag
     1681 aacatgacta tttcaagggg gggactgatg cagtgtgagg aattgatagc gtatctgcgg
     1741 gatgaatctg aatttagaga caaactcact ccaattacta tttttatgga atatcggttg
     1801 gattatagaa cagctgctga tacaacaggc ttgcaaccca ttcttaacca gttcacgcct
     1861 gctaacatta gtcgacaggc tcacattcta cttgactgtg gtgaagacaa tgtctgtaaa
     1921 cccaagctgg aagtttctgt agatagtgat caaaagaaga tctatattgg ggatgacaac
     1981 cctctgacat tgattgttaa ggctcagaat caaggagaag gtgcctacga agctgagctc
     2041 atcgtttcca ttccactgca ggctgatttc atcggggttg tccgaaacaa tgaagcctta
     2101 gcaagacttt cctgtgcatt taagacagaa aaccaaactc gccaggtggt atgtgacctt
     2161 ggaaacccaa tgaaggctgg aactcaactc ttagctggtc ttcgtttcag tgtgcaccag
     2221 cagtcagaga tggatacttc tgtgaaattt gacttacaaa tccaaagctc aaatctattt
     2281 gacaaagtaa gcccagttgt atctcacaaa gttgatcttg ctgttttagc tgcagttgag
     2341 ataagaggag tctcgagtcc tgatcatatc tttcttccga ttccaaactg ggagcacaag
     2401 gagaaccctg agactgaaga agatgttggg ccagttgttc agcacatcta tgagctgaga
     2461 aacaatggtc caagttcatt cagcaaggca atgctccatc ttcagtggcc ttacaaatat
     2521 aataataaca ctctgttgta tatccttcat tatgatattg atggaccaat gaactgcact
     2581 tcagatatgg agatcaaccc tttgagaatt aagatctcat ctttgcaaac aactgaaaag
     2641 aatgacacgg ttgccgggca aggtgagcgg gaccatctca tcactaagcg ggatcttgcc
     2701 ctcagtgaag gagatattca cactttgggt tgtggagttg ctcagtgctt gaagattgtc
     2761 tgccaagttg ggagattaga cagaggaaag agtgcaatct tgtacgtaaa gtcattactg
     2821 tggactgaga cttttatgaa taaagaaaat cagaatcatt cctattctct gaagtcgtct
     2881 gcttcattta atgtcataga gtttccttat aagaatcttc caattgagga tatcaccaac
     2941 tccacattgg ttaccactaa tgtcacctgg ggcattcagc cagcgcccat gcctgtgcct
     3001 gtgtgggtga tcattttagc agttctagca ggattgttgc tactggctgt tttggtattt
     3061 gtaatgtaca ggatgggctt ttttaaacgg gtccggccac ctcaagaaga acaagaaagg
     3121 gagcagcttc aacctcatga aaatggtgaa ggaaactcag aaactccggg atctcgagct
     3181 caagcttcga attctgcagt cgacggtacc gcgggcccgg gatccccacc ggtcgccacc
     3241 atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac
     3301 ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac
     3361 ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc
     3421 ctcgtgacca ccttgaccta cggcgtgcag tgcttcgccc gctaccccga ccacatgaag
     3481 cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc
     3541 ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg
     3601 gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac
     3661 aagctggagt acaactacaa cagccacaag gtctatatca ccgccgacaa gcagaagaac
     3721 ggcatcaagg tgaacttcaa gacccgccac aacatcgagg acggcagcgt gcagctcgcc
     3781 gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac
     3841 tacctgagca cccagtccaa gctgagcaaa gaccccaacg agaagcgcga tcacatggtc
     3901 ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagtaa
     3961 gcggccgcga ctctagatca taatcagcca taccaca
//
