LOCUS       sequence-84179-e        1965 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Actin, Excitation: 487, Emission: 509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1965)
  TITLE     Davidson mEmerald
REFERENCE   2  (bases 1 to 1965)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEmerald-Alpha-Actin-12
FEATURES             Location/Qualifiers
     source          1..1965
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      29..38
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(35..37,38..40,41..751)
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(80..101)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(341..360)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     688..709
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gacgtcagat ccgctagcgc taccggtcgc caccatggtg agcaagggcg aggagctgtt
       61 caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag
      121 cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg
      181 caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccttga cctacggcgt
      241 gcagtgcttc gcccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat
      301 gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac
      361 ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat
      421 cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca
      481 caaggtctat atcaccgccg acaagcagaa gaacggcatc aaggtgaact tcaagacccg
      541 ccacaacatc gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat
      601 cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agcacccagt ccaagctgag
      661 caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg
      721 gatcactctc ggcatggacg agctgtacaa gtccggactc agatctcgag ctcaagcttc
      781 gaattctatg tgtgaagaag aggacagcac tgccttggtg tgtgacaatg gctctgggct
      841 ctgtaaggcc ggctttgctg gggacgatgc tcccagggct gttttcccat ccattgtggg
      901 acgtcccaga catcaggggg tgatggtggg aatgggacaa aaagacagct acgtgggtga
      961 cgaagcacag agcaaaagag gaatcctgac cctgaagtac ccgatagaac atggcatcat
     1021 caccaactgg gacgacatgg aaaagatctg gcaccactct ttctacaatg agcttcgtgt
     1081 tgcccctgaa gagcatccca ccctgctcac ggaggcaccc ctgaacccca aggccaaccg
     1141 ggagaaaatg actcaaatta tgtttgagac tttcaatgtc ccagccatgt atgtggctat
     1201 ccaggcggtg ctgtctctct atgcctctgg acgcacaact ggcatcgtgc tggactctgg
     1261 agatggtgtc acccacaatg tccccatcta tgagggctat gccttgcccc atgccatcat
     1321 gcgtctggat ctggctggcc gagatctcac tgactacctc atgaagatcc tgactgagcg
     1381 tggctattcc ttcgttacta ctgctgagcg tgagattgtc cgggacatca aggagaaact
     1441 gtgttatgta gctctggact ttgaaaatga gatggccact gccgcatcct catcctccct
     1501 tgagaagagt tacgagttgc ctgatgggca agtgatcacc atcggaaatg aacgtttccg
     1561 ctgcccagag accctgttcc agccatcctt catcgggatg gagtctgctg gcatccatga
     1621 aaccacctac aacagcatca tgaagtgtga tattgacatc aggaaggacc tctatgctaa
     1681 caatgtccta tcagggggca ccactatgta ccctggcatt gccgaccgaa tgcagaagga
     1741 gatcacggcc ctagcaccca gcaccatgaa gatcaagatc attgcccctc cggagcgcaa
     1801 atactctgtc tggatcggtg gctccatcct ggcctctctg tccaccttcc agcagatgtg
     1861 gatcagcaaa caggaatacg atgaagccgg gccttccatc gtccaccgca aatgcttcta
     1921 aggatccacc ggatctagat aactgatcat aatcagccat accac
//
