LOCUS       sequence-84180-e        1987 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Actin, Excitation: 487, Emission: 509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1987)
  TITLE     Davidson mEmerald
REFERENCE   2  (bases 1 to 1987)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEmerald-Alpha-Actin2-C-18
FEATURES             Location/Qualifiers
     source          1..1987
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      22..31
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(28..30,31..33,34..744)
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(73..94)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(334..353)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     681..702
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gatccgctag cgctaccggt cgccaccatg gtgagcaagg gcgaggagct gttcaccggg
       61 gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc
      121 ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc
      181 ggcaagctgc ccgtgccctg gcccaccctc gtgaccacct tgacctacgg cgtgcagtgc
      241 ttcgcccgct accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa
      301 ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc
      361 gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc
      421 aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaaggtc
      481 tatatcaccg ccgacaagca gaagaacggc atcaaggtga acttcaagac ccgccacaac
      541 atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac
      601 ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccaagct gagcaaagac
      661 cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact
      721 ctcggcatgg acgagctgta caagtccgga ctcagatctc gaggagggtc cagcggcggt
      781 ggatctgggt ccagcggatg tgaagaggaa gacagcacag ccctggtgtg cgacaatggt
      841 tctgggctct gtaaggccgg cttcgctggt gatgatgctc ccagggctgt tttcccatcc
      901 atcgtgggac gtcccagaca tcagggagta atggttggaa tgggccaaaa agacagctat
      961 gtgggggatg aagcccagag caagagaggg atcctgacgc tgaagtatcc gatagaacac
     1021 ggcatcatca ccaactggga cgacatggaa aagatctggc accactcttt ctataacgag
     1081 cttcgtgtgg cccctgaaga gcatccgaca ctgctgacag aggcaccact gaaccctaag
     1141 gccaaccggg agaaaatgac ccagattatg tttgagacct tcaatgtccc cgccatgtat
     1201 gtggctattc aggctgtgct gtccctctat gcctctggac gtacaactgg tattgtgctg
     1261 gactctggag atggtgtgac tcacaacgtg cctatctatg agggctatgc cctgcctcat
     1321 gccatcatgc gtctggactt ggctggccga gatctcaccg actacctcat gaagatcctg
     1381 actgagcgtg gctattcctt cgtgactact gccgagcgtg agattgtccg tgacatcaag
     1441 gagaagctgt gctatgtagc tctggacttt gaaaatgaga tggccacggc cgcctcctct
     1501 tcctccctgg agaagagcta cgaactgcct gacgggcagg tgatcaccat tggaaacgaa
     1561 cgcttccgct gcccagagac tctcttccag ccatctttca ttgggatgga gtcagcgggc
     1621 atccacgaaa ccacctataa cagcatcatg aagtgtgata ttgacatcag gaaggatctc
     1681 tatgctaaca acgtcctgtc agggggtacc accatgtacc caggcattgc tgacaggatg
     1741 cagaaggaga tcacagccct cgcacccagc accatgaaga tcaagatcat tgcccctcca
     1801 gaacgcaagt actctgtctg gatcggtggc tccatcctgg cttcgctgtc taccttccag
     1861 cagatgtgga tcagcaaaca ggaatacgac gaagctgggc cctccatcgt ccaccgcaaa
     1921 tgcttctaag aattctgcag tcgacggtac cgcgggcccg ggatccaccg gatctagata
     1981 actgatc
//
