LOCUS       sequence-84183-e        3542 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Non-muscle Alpha Actinin, Excitation: 487, Emission: 
            509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3542)
  TITLE     Davidson mEmerald
REFERENCE   2  (bases 1 to 3542)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEmerald-Alpha-Actinin-C-14
FEATURES             Location/Qualifiers
     source          1..3542
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      28..37
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(34..36,37..39,40..750)
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(79..100)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(340..359)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     687..708
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 acgtcagatc cgctagcgct accggtcgcc accatggtga gcaagggcga ggagctgttc
       61 accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc
      121 gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc
      181 accaccggca agctgcccgt gccctggccc accctcgtga ccaccttgac ctacggcgtg
      241 cagtgcttcg cccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg
      301 cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc
      361 cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc
      421 gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta caacagccac
      481 aaggtctata tcaccgccga caagcagaag aacggcatca aggtgaactt caagacccgc
      541 cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc
      601 ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc caagctgagc
      661 aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg
      721 atcactctcg gcatggacga gctgtacaag tccggactca gatctcgagg aagcggcgga
      781 tctgggtccg gagaccatta tgattctcag caaaccaacg attacatgca gccagaagag
      841 gactgggacc gggacctgct cctggacccg gcctgggaga agcagcagag aaagacattc
      901 acggcatggt gtaactccca cctccggaag gcggggacac agatcgagaa catcgaagag
      961 gacttccggg atggcctgaa gctcatgctg ctgctggagg tcatctcagg tgaacgcttg
     1021 gccaagccag agcgaggcaa gatgagagtg cacaagatct ccaacgtcaa caaggccctg
     1081 gatttcatag ccagcaaagg cgtcaaactg gtgtccatcg gagccgaaga aatcgtggat
     1141 gggaatgtga agatgaccct gggcatgatc tggaccatca tcctgcgctt tgccatccag
     1201 gacatctccg tggaagagac ttcagccaag gaagggctgc tcctgtggtg tcagagaaag
     1261 acagcccctt acaaaaatgt caacatccag aacttccaca taagctggaa ggatggcctc
     1321 ggcttctgtg ctttgatcca ccgacaccgg cccgagctga ttgactacgg gaagctgcgg
     1381 aaggatgatc cactcacaaa tctgaatacg gcttttgacg tggcagagaa gtacctggac
     1441 atccccaaga tgctggatgc cgaagacatc gttggaactg cccgaccgga tgagaaagcc
     1501 atcatgactt acgtgtctag cttctaccac gccttctctg gagcccagaa ggcggagaca
     1561 gcagccaatc gcatctgcaa ggtgttggcc gtcaaccagg agaacgagca gcttatggaa
     1621 gactacgaga agctggccag tgatctgttg gagtggatcc gccgcacaat cccgtggctg
     1681 gagaaccggg tgcccgagaa caccatgcat gccatgcaac agaagctgga ggacttccgg
     1741 gactaccggc gcctgcacaa gccgcccaag gtgcaggaga agtgccagct ggagatcaac
     1801 ttcaacacgc tgcagaccaa gctgcggctc agcaaccggc ctgccttcat gccctctgag
     1861 ggcaggatgg tctcggacat caacaatgcc tggggctgcc tggagcaggt ggagaagggc
     1921 tatgaggagt ggttgctgaa tgagatccgg aggctggagc gactggacca cctggcagag
     1981 aagttccggc agaaggcctc catccacgag gcctggactg acggcaaaga ggccatgctg
     2041 cgacagaagg actatgagac cgccaccctc tcggagatca aggccttgct caagaagcat
     2101 gaggccttcg agagtgacct ggctgcccac caggaccgtg tggagcagat tgccgccatc
     2161 gcacaggagc tcaatgagct ggactattat gactcaccca gtgtcaacgc ccgttgccaa
     2221 aagatctgtg accagtggga caatctgggg gccctaactc agaagcgaag ggaagctctg
     2281 gagcggaccg agaaactgct ggagaccatt gaccagctgt acttggagta tgccaagcgg
     2341 gctgcaccct tcaacaactg gatggagggg gccatggagg acctgcagga caccttcatt
     2401 gtgcacacca ttgaggagat ccagggactg accacagccc atgagcagtt caaggccacc
     2461 ctccctgatg ccgacaagga gcgcctggcc atcctgggca tccacaatga ggtgtccaag
     2521 attgtccaga cctaccacgt caatatggcg ggcaccaacc cctacacaac catcacgcct
     2581 caggagatca atggcaaatg ggaccacgtg cggcagctgg tgcctcggag ggaccaagct
     2641 ctgacggagg agcatgcccg acagcagcac aatgagaggc tacgcaagca gtttggagcc
     2701 caggccaatg tcatcgggcc ctggatccag accaagatgg aggagatcgg gaggatctcc
     2761 attgagatgc atgggaccct ggaggaccag ctcagccacc tgcggcagta tgagaagagc
     2821 atcgtcaact acaagccaaa gattgatcag ctggagggcg accaccagct catccaggag
     2881 gcgctcatct tcgacaacaa gcacaccaac tacaccatgg agcacatccg tgtgggctgg
     2941 gagcagctgc tcaccaccat cgccaggacc atcaatgagg tagagaacca gatcctgacc
     3001 cgggatgcca agggcatcag ccaggagcag atgaatgagt tccgggcctc cttcaaccac
     3061 tttgaccggg atcactccgg cacactgggt cccgaggagt tcaaagcctg cctcatcagc
     3121 ttgggttatg atattggcaa cgacccccag ggagaagcag aatttgcccg catcatgagc
     3181 attgtggacc ccaaccgcct gggggtagtg acattccagg ccttcattga cttcatgtcc
     3241 cgcgagacag ccgacacaga tacagcagac caagtcatgg cttccttcaa gatcctggct
     3301 ggggacaaga actacattac catggacgag ctgcgccgcg agctgccacc cgaccaggct
     3361 gagtactgca tcgcgcggat ggccccctac accggccccg actccgtgcc aggtgctctg
     3421 gactacatgt ccttctccac ggcgctgtac ggcgagagtg acctctaaga attctgcagt
     3481 cgacggtacc gcgggcccgg gatccaccgg atctagataa ctgatcataa tcagccatac
     3541 ca
//
