LOCUS       sequence-84184-e        1800 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Plasma Membrane, Excitation: 487, Emission: 509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1800)
  TITLE     Davidson mEmerald
REFERENCE   2  (bases 1 to 1800)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEmerald-Annexin-12
FEATURES             Location/Qualifiers
     source          1..1800
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      29..38
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(35..37,38..40,41..751)
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(80..101)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(341..360)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     688..709
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gacgtcagat ccgctagcgc taccggtcgc caccatggtg agcaagggcg aggagctgtt
       61 caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag
      121 cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg
      181 caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccttga cctacggcgt
      241 gcagtgcttc gcccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat
      301 gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac
      361 ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat
      421 cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca
      481 caaggtctat atcaccgccg acaagcagaa gaacggcatc aaggtgaact tcaagacccg
      541 ccacaacatc gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat
      601 cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agcacccagt ccaagctgag
      661 caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg
      721 gatcactctc ggcatggacg agctgtacaa gtccggactc agatctcgag ctcaagcttc
      781 gaattctatg gccatggcaa ccaaaggagg tactgtcaaa gctgcttcag gattcaatgc
      841 catggaagat gcccagaccc tgaggaaggc catgaaaggg ctcggcaccg atgaagacgc
      901 cattattagc gtccttgcct accgcaacac cgcccagcgc caggagatca ggacagccta
      961 caagagcacc atcggcaggg acttgataga cgacctgaag tcagaactga gtggcaactt
     1021 cgagcaggtg attgtgggga tgatgacgcc cacggtgctg tatgacgtgc aagagctgcg
     1081 aagggccatg aagggagccg gcactgatga gggctgccta attgagatcc tggcctcccg
     1141 gacccctgag gagatccggc gcataagcca aacctaccag cagcaatatg gacggagcct
     1201 tgaagatgac attcgctctg acacatcgtt catgttccag cgagtgctgg tgtctctgtc
     1261 agctggtggg agggatgaag gaaattatct ggacgatgct ctcgtgagac aggatgccca
     1321 ggacctgtat gaggctggag agaagaaatg ggggacagat gaggtgaaat ttctaactgt
     1381 tctctgttcc cggaaccgaa atcacctgtt gcatgtgttt gatgaataca aaaggatatc
     1441 acagaaggat attgaacaga gtattaaatc tgaaacatct ggtagctttg aagatgctct
     1501 gctggctata gtaaagtgca tgaggaacaa atctgcatat tttgctgaaa agctctataa
     1561 atcgatgaag ggcttgggca ccgatgataa caccctcatc agagtgatgg tttctcgagc
     1621 agaaattgac atgttggata tccgggcaca cttcaagaga ctctatggaa agtctctgta
     1681 ctcgttcatc aagggtgaca catctggaga ctacaggaaa gtactgcttg ttctctgtgg
     1741 aggagatgat taaggatcca ccggatctag ataactgatc ataatcagcc ataccacatt
//
