LOCUS       sequence-84185-e        2029 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Actin Related Protein, Excitation: 487, Emission: 509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2029)
  TITLE     Davidson mEmerald
REFERENCE   2  (bases 1 to 2029)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEmerald-ARP2-C-14
FEATURES             Location/Qualifiers
     source          1..2029
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      24..33
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(30..32,33..35,36..746)
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(75..96)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(336..355)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     683..704
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 cagatccgct agcgctaccg gtcgccacca tggtgagcaa gggcgaggag ctgttcaccg
       61 gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt
      121 ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca
      181 ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cttgacctac ggcgtgcagt
      241 gcttcgcccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg
      301 aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg
      361 ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact
      421 tcaaggagga cggcaacatc ctggggcaca agctggagta caactacaac agccacaagg
      481 tctatatcac cgccgacaag cagaagaacg gcatcaaggt gaacttcaag acccgccaca
      541 acatcgagga cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg
      601 acggccccgt gctgctgccc gacaaccact acctgagcac ccagtccaag ctgagcaaag
      661 accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca
      721 ctctcggcat ggacgagctg tacaagtccg gaagtgctgg gagcgcaagc ggaagcggag
      781 gctccggtga cagccagggc aggaaggtgg tggtgtgcga caacggcacc gggtttgtga
      841 agtgtggata tgcaggctct aactttccag aacacatctt cccagctttg gttggaagac
      901 ctattatcag atcaaccacc aaagtgggaa acattgaaat caagaataac aaaaagatgg
      961 atcttatggt tggtgatgag gcaagtgaat tacgatcaat gttagaagtt aactacccta
     1021 tggaaaatgg catagtacga aattgggatg acatgaaaca cctgtgggac tacacatttg
     1081 gaccagagaa acttaatata gataccagaa attgtaaaat cttactcaca gaacctccta
     1141 tgaacccaac caaaaacaga gagaagattg tagaggtaat gtttgaaact taccagtttt
     1201 ccggtgtata tgtagccatc caggcagttc tgactttgta cgctcaaggt ttattgactg
     1261 gtgtagtggt agactctgga gatggtgtga ctcacatttg cccagtatat gaaggctttt
     1321 ctctccctca tcttaccagg agactggata ttgctgggag ggatataact agatatctta
     1381 tcaagctact tctgttgcga ggatacgcct tcaaccactc tgctgatttt gaaacggttc
     1441 gcatgattaa agaaaaactg tgttacgtgg gatataatat tgagcaagag cagaaactgg
     1501 ccttagaaac cacagtatta gttgaatctt atacactccc agatggacgt atcatcaaag
     1561 ttgggggaga gagatttgaa gcaccagaag ctttatttca gcctcacttg atcaatgttg
     1621 aaggagttgg tgttgctgaa ttgcttttta acacaattca ggcagctgac attgatacca
     1681 gatctgaatt ctacaaacac attgtgcttt ctggagggtc tactatgtat cctggcctgc
     1741 catcacggtt ggaacgagaa cttaaacagc tttacttaga acgagttttg aagggtgatg
     1801 tggaaaaact ttctaaattt aagatccgca ttgaagaccc accccgcaga aagcacatgg
     1861 tattcctggg tggtgcagtt ctagcggata tcatgaaaga caaagacaac ttttggatga
     1921 cccgacaaga gtaccaagaa aagggtgtcc gtgtgctaga gaaacttggt gtgactgttc
     1981 gataaggatc caccggatct agataactga tcataatcag ccataccac
//
