LOCUS       sequence-84186-e        2036 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Actin Related Protein, Excitation: 487, Emission: 509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2036)
  TITLE     Davidson mEmerald
REFERENCE   2  (bases 1 to 2036)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEmerald-ARP2-N-14
FEATURES             Location/Qualifiers
     source          1..2036
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      1275..1284
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1281..1283,1284..1286,1287..2000)
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1326..1347)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1587..1606)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1934..1955
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 cagatccgct agcgctaccg gactcagatc tcgagaccgc catggacagc cagggcagga
       61 aggtggtggt gtgcgacaac ggcaccgggt ttgtgaagtg tggatatgca ggctctaact
      121 ttccagaaca catcttccca gctttggttg gaagacctat tatcagatca accaccaaag
      181 tgggaaacat tgaaatcaag aataacaaaa agatggatct tatggttggt gatgaggcaa
      241 gtgaattacg atcaatgtta gaagttaact accctatgga aaatggcata gtacgaaatt
      301 gggatgacat gaaacacctg tgggactaca catttggacc agagaaactt aatatagata
      361 ccagaaattg taaaatctta ctcacagaac ctcctatgaa cccaaccaaa aacagagaga
      421 agattgtaga ggtaatgttt gaaacttacc agttttccgg tgtatatgta gccatccagg
      481 cagttctgac tttgtacgct caaggtttat tgactggtgt agtggtagac tctggagatg
      541 gtgtgactca catttgccca gtatatgaag gcttttctct ccctcatctt accaggagac
      601 tggatattgc tgggagggat ataactagat atcttatcaa gctacttctg ttgcgaggat
      661 acgccttcaa ccactctgct gattttgaaa cggttcgcat gattaaagaa aaactgtgtt
      721 acgtgggata taatattgag caagagcaga aactggcctt agaaaccaca gtattagttg
      781 aatcttatac actcccagat ggacgtatca tcaaagttgg gggagagaga tttgaagcac
      841 cagaagcttt atttcagcct cacttgatca atgttgaagg agttggtgtt gctgaattgc
      901 tttttaacac aattcaggca gctgacattg ataccagatc tgaattctac aaacacattg
      961 tgctttctgg agggtctact atgtatcctg gcctgccatc acggttggaa cgagaactta
     1021 aacagcttta cttagaacga gttttgaagg gtgatgtgga aaaactttct aaatttaaga
     1081 tccgcattga agacccaccc cgcagaaagc acatggtatt cctgggtggt gcagttctag
     1141 cggatatcat gaaagacaaa gacaactttt ggatgacccg acaagagtac caagaaaagg
     1201 gtgtccgtgt gctagagaaa cttggtgtga ctgttcgagg gggtgcgtca ggcggcagcg
     1261 gatctggacc ggtcgccacc atggtgagca agggcgagga gctgttcacc ggggtggtgc
     1321 ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg
     1381 gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc
     1441 tgcccgtgcc ctggcccacc ctcgtgacca ccttgaccta cggcgtgcag tgcttcgccc
     1501 gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg
     1561 tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga
     1621 agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg
     1681 acggcaacat cctggggcac aagctggagt acaactacaa cagccacaag gtctatatca
     1741 ccgccgacaa gcagaagaac ggcatcaagg tgaacttcaa gacccgccac aacatcgagg
     1801 acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg
     1861 tgctgctgcc cgacaaccac tacctgagca cccagtccaa gctgagcaaa gaccccaacg
     1921 agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca
     1981 tggacgagct gtacaagtaa gcggccgcga ctctagatca taatcagcca taccac
//
