LOCUS       sequence-84188-e        2059 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Actin Related Protein, Excitation: 487, Emission: 509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2059)
  TITLE     Davidson mEmerald
REFERENCE   2  (bases 1 to 2059)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEmerald-ARP3-N-12
FEATURES             Location/Qualifiers
     source          1..2059
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      20..29
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      1310..1319
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1316..1318,1319..1321,1322..2035)
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1361..1382)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1622..1641)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1969..1990
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gacgtcagat ccgctagcag ccaccatggc gggacggctg ccggcctgtg tggtggactg
       61 tggcacgggg tatacaaaac taggatatgc tggaaataca gaaccacagt ttatcatccc
      121 ttcctgtatt gctattaagg agtcagcaaa agtgggtgat caagctcaaa ggagggtgat
      181 gaaaggtgtt gatgacctag acttcttcat tggtgatgaa gcaatagaaa aacctacata
      241 tgcaacaaag tggccaatcc gccatggtat agttgaagat tgggacttaa tggaaaggtt
      301 tatggagcaa gtgatcttta aatatttaag ggcagaacct gaagaccatt attttctttt
      361 gactgaacct ccattgaata ctccagaaaa cagggaatat actgctgaaa taatgtttga
      421 gtccttcaat gttccaggct tgtacattgc tgtgcaggct gttcttgcct tagctgcatc
      481 ttggacctca agacaagtag gagaacggac gttgaccggt acggtaatag acagtggaga
      541 tggtgtcact catgtcattc ctgtggctga agggtatgtg attggcagct gtattaaaca
      601 cattccaatc gcaggacgag atataacata ttttattcag caactgctga gagaccgaga
      661 agtaggaatc cctccagaac aatccttgga aactgctaag gcagtaaagg agcgctatag
      721 ttatgtctgc ccagatttag taaaagaatt taacaagtat gatacagatg ggtcaaaatg
      781 gattaaacag tatactggaa tcaatgctat ctcaaagaaa gagttttcta tcgatgttgg
      841 ttatgagaga tttttgggac ctgaaatctt ttttcatcca gagtttgcta atccagactt
      901 tacacaacct atctcagaag ttgtagatga agtaattcag aattgtccta ttgatgtcag
      961 acgtcctctc tacaagaata ttgtcctctc tggaggttca accatgttca gggactttgg
     1021 acgtcgcttg caaagagatt tgaaaagaac tgtagatgcc cggctgaaat taagtgagga
     1081 attgagtggt ggtagattga agccaaaacc tattgatgta caagtcatta cacaccacat
     1141 gcagcgatat gcagtttggt ttggaggatc aatgctggct tccacgcctg agttctacca
     1201 agtatgccac accaaaaagg attatgaaga aattggacct agcatttgtc gtcacaatcc
     1261 agtgtttgga gtcatgtcgg catccggcgg tagcggggat ccaccggtcg ccaccatggt
     1321 gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga
     1381 cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa
     1441 gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt
     1501 gaccaccttg acctacggcg tgcagtgctt cgcccgctac cccgaccaca tgaagcagca
     1561 cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa
     1621 ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa
     1681 ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct
     1741 ggagtacaac tacaacagcc acaaggtcta tatcaccgcc gacaagcaga agaacggcat
     1801 caaggtgaac ttcaagaccc gccacaacat cgaggacggc agcgtgcagc tcgccgacca
     1861 ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct
     1921 gagcacccag tccaagctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct
     1981 ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca agtaagcggc
     2041 cgcgactcta gatcataat
//
