LOCUS       sequence-84189-e        1730 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Actin Related Protein, Excitation: 487, Emission: 509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1730)
  TITLE     Davidson mEmerald
REFERENCE   2  (bases 1 to 1730)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEmerald-ARPp34-C-14
FEATURES             Location/Qualifiers
     source          1..1730
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      22..31
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(28..30,31..33,34..744)
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(73..94)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(334..353)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     681..702
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gatccgctag cgctaccggt cgccaccatg gtgagcaagg gcgaggagct gttcaccggg
       61 gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc
      121 ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc
      181 ggcaagctgc ccgtgccctg gcccaccctc gtgaccacct tgacctacgg cgtgcagtgc
      241 ttcgcccgct accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa
      301 ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc
      361 gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc
      421 aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaaggtc
      481 tatatcaccg ccgacaagca gaagaacggc atcaaggtga acttcaagac ccgccacaac
      541 atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac
      601 ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccaagct gagcaaagac
      661 cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact
      721 ctcggcatgg acgagctgta caagtccgga agtgctggga gcgcaagcgg aagcggaggc
      781 tccggtatcc tgctggaggt gaacaaccgc atcatcgagg agacgctcgc gctcaagttc
      841 gagaacgcag ccgccggaaa caaaccggaa gcagtagaag taacatttgc agatttcgat
      901 ggggtcctct atcatatttc aaatcctaat ggagacaaaa caaaagtgat ggtcagtatt
      961 tctttgaaat tctacaagga acttcaggca catggtgctg atgagttatt aaagagggtg
     1021 tacgggagtt tcttggtaaa tccagaatca ggatacaatg tctctttgct atatgacctt
     1081 gaaaatcttc cggcatccaa ggattccatt gtgcatcaag ctggcatgtt gaagcgaaat
     1141 tgttttgcct ctgtctttga aaaatacttc caattccaag aagagggcaa ggaaggagag
     1201 aacagggcag ttatccatta tagggatgat gagaccatgt atgttgagtc taaaaaggac
     1261 agagtcacag tagtcttcag cacagtgttt aaggatgacg acgatgtggt cattggaaag
     1321 gtgttcatgc aggagttcaa agaaggacgc agagccagcc acacagcccc acaggtcctc
     1381 tttagccaca gggaacctcc tctggagctg aaagacacag acgccgctgt gggtgacaac
     1441 attggctaca ttacctttgt gctgttccct cgtcacacca atgccagtgc acgagacaac
     1501 accatcaacc tgatccacac gttccgggac tacctgcact accacatcaa gtgctctaag
     1561 gcctatattc acacacgtat gcgggcgaaa acgtctgact tcctcaaggt gctgaaccgc
     1621 gcacgcccag atgccgagaa aaaagaaatg aaaacaatca cggggaagac gttttcatcc
     1681 cgctaaggat ccaccggatc tagataactg atcataatca gccataccac
//
