LOCUS       sequence-84190-e        1738 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Actin Related Protein, Excitation: 487, Emission: 509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1738)
  TITLE     Davidson mEmerald
REFERENCE   2  (bases 1 to 1738)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEmerald-ARPp34-N-14
FEATURES             Location/Qualifiers
     source          1..1738
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      976..985
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(982..984,985..987,988..1701)
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1027..1048)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1288..1307)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1635..1656
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gatccgctag cgctaccgga ctcagatctc gagaccgcca tgatcctgct ggaggtgaac
       61 aaccgcatca tcgaggagac gctcgcgctc aagttcgaga acgcagccgc cggaaacaaa
      121 ccggaagcag tagaagtaac atttgcagat ttcgatgggg tcctctatca tatttcaaat
      181 cctaatggag acaaaacaaa agtgatggtc agtatttctt tgaaattcta caaggaactt
      241 caggcacatg gtgctgatga gttattaaag agggtgtacg ggagtttctt ggtaaatcca
      301 gaatcaggat acaatgtctc tttgctatat gaccttgaaa atcttccggc atccaaggat
      361 tccattgtgc atcaagctgg catgttgaag cgaaattgtt ttgcctctgt ctttgaaaaa
      421 tacttccaat tccaagaaga gggcaaggaa ggagagaaca gggcagttat ccattatagg
      481 gatgatgaga ccatgtatgt tgagtctaaa aaggacagag tcacagtagt cttcagcaca
      541 gtgtttaagg atgacgacga tgtggtcatt ggaaaggtgt tcatgcagga gttcaaagaa
      601 ggacgcagag ccagccacac agccccacag gtcctcttta gccacaggga acctcctctg
      661 gagctgaaag acacagacgc cgctgtgggt gacaacattg gctacattac ctttgtgctg
      721 ttccctcgtc acaccaatgc cagtgcacga gacaacacca tcaacctgat ccacacgttc
      781 cgggactacc tgcactacca catcaagtgc tctaaggcct atattcacac acgtatgcgg
      841 gcgaaaacgt ctgacttcct caaggtgctg aaccgcgcac gcccagatgc cgagaaaaaa
      901 gaaatgaaaa caatcacggg gaagacgttt tcatcccgcg ggggtgcgtc aggcggcagc
      961 ggatctggac cggtcgccac catggtgagc aagggcgagg agctgttcac cggggtggtg
     1021 cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag
     1081 ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag
     1141 ctgcccgtgc cctggcccac cctcgtgacc accttgacct acggcgtgca gtgcttcgcc
     1201 cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac
     1261 gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg
     1321 aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag
     1381 gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa ggtctatatc
     1441 accgccgaca agcagaagaa cggcatcaag gtgaacttca agacccgcca caacatcgag
     1501 gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc
     1561 gtgctgctgc ccgacaacca ctacctgagc acccagtcca agctgagcaa agaccccaac
     1621 gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc
     1681 atggacgagc tgtacaagta agcggccgcg actctagatc ataatcagcc atacccac
//
