LOCUS       sequence-84192-e        1767 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Membrane Receptor, Excitation: 487, Emission: 509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1767)
  TITLE     Davidson mEmerald
REFERENCE   2  (bases 1 to 1767)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEmerald-ATG3-N-18
FEATURES             Location/Qualifiers
     source          1..1767
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      1013..1022
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1019..1021,1022..1024,1025..1738)
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1064..1085)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1325..1344)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1672..1693
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gctagcgcta ccggactcag atctcgaggc caccatgcag aatgtgatca acacggtgaa
       61 gggaaaggct ctggaagtgg ccgagtacct gaccccggtc ctcaaggaat caaaatttaa
      121 ggaaacgggt gtaatcactc cagaagagtt tgtggcagct ggagatcact tagtccacca
      181 ctgtccaaca tggcaatggg ctacagggga agaattgaaa gtgaaggcat atcttccgac
      241 agacaaacaa tttttggtaa ccaaaaatgt tccatgctac aagcggtgta aacagatgga
      301 gtattcggat gaattggaag ctatcattga agaagatgat ggtgatgggg gatgggtaga
      361 tacatatcac aacacaggta ttacaggaat tactgaagca gttaaggaga ttacactgga
      421 aagcaaggac agtataaaac tccaagattg ctcagcactg tgtgatgaag aagacgagga
      481 agatgaaggg gaagctgcag acatggaaga atatgaagag agtggattgt tggaaacaga
      541 tgaggctacc ctagacacaa ggaaaatagt ggaagcctgc aaagctaagg ctgacgctgg
      601 aggtgaagat gctattttac aaacgagaac atacgatctg tacatcactt acgacaaata
      661 ttaccagaca ccacggctat ggttgtttgg ctatgatgag caacggcagc ctttaacagt
      721 tgagcacatg tatgaagaca tcagtcaaga tcatgtgaag aaaacagtga ccattgaaaa
      781 ccatcctcat ctcccaccac ctcctatgtg ttcagttcac ccatgcaggc atgctgaagt
      841 gatgaagaaa attattgaga cagttgcaga aggcggggga gagcttggtg ttcatatgta
      901 tcttttaatt tttttgaaat ttgttcaagc tgtcattcca acaatagaat atgactacac
      961 aagacacttc acaatgggca gcgcatccgg cggaagcgga agcggaccgg tcgccaccat
     1021 ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg
     1081 cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg
     1141 caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct
     1201 cgtgaccacc ttgacctacg gcgtgcagtg cttcgcccgc taccccgacc acatgaagca
     1261 gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt
     1321 caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt
     1381 gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa
     1441 gctggagtac aactacaaca gccacaaggt ctatatcacc gccgacaagc agaagaacgg
     1501 catcaaggtg aacttcaaga cccgccacaa catcgaggac ggcagcgtgc agctcgccga
     1561 ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta
     1621 cctgagcacc cagtccaagc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct
     1681 gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagtaagc
     1741 ggccgcgact ctagatcata atcagcc
//
