LOCUS       sequence-84193-e        1683 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Membrane Receptor, Excitation: 487, Emission: 509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1683)
  TITLE     Davidson mEmerald
REFERENCE   2  (bases 1 to 1683)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEmerald-ATG5-C-18
FEATURES             Location/Qualifiers
     source          1..1683
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      25..34
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(31..33,34..36,37..747)
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(76..97)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(337..356)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     684..705
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 tcagatccgc tagcgctacc ggtcgccacc atggtgagca agggcgagga gctgttcacc
       61 ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg
      121 tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc
      181 accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccttgaccta cggcgtgcag
      241 tgcttcgccc gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc
      301 gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc
      361 gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac
      421 ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa cagccacaag
      481 gtctatatca ccgccgacaa gcagaagaac ggcatcaagg tgaacttcaa gacccgccac
      541 aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc
      601 gacggccccg tgctgctgcc cgacaaccac tacctgagca cccagtccaa gctgagcaaa
      661 gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc
      721 actctcggca tggacgagct gtacaagtcc ggactcagat ctcgaggaag cggcggatct
      781 gggtccggca gctccggcag cacagatgac aaagatgtgc ttcgagatgt gtggtttgga
      841 cgaattccaa cttgtttcac gctatatcag gatgagataa ctgaaaggga agcagaacca
      901 tactatttgc ttttgccaag agtaagttat ttgacgttgg tgactgacaa agtgaaaaag
      961 cactttcaga aggttatgag acaagaagac attagtgaga tatggtttga atatgaaggc
     1021 acaccactga aatggcatta tccaattggt ttgctatttg atcttcttgc atcaagttca
     1081 gctcttcctt ggaacatcac agtacatttt aagagttttc cagaaaaaga ccttctgcac
     1141 tgtccatcta aggatgcaat tgaagctcat tttatgtcat gtatgaaaga agctgatgct
     1201 ttaaaacata aaagtcaagt aatcaatgaa atgcagaaaa aagatcacaa gcaactctgg
     1261 atgggattgc aaaatgacag atttgaccag ttttgggcca tcaatcggaa actcatggaa
     1321 tatcctgcag aagaaaatgg atttcgttat atccccttta gaatatatca gacaacgact
     1381 gaaagacctt tcattcagaa gctgtttcgt cctgtggctg cagatggaca gttgcacaca
     1441 ctaggagatc tcctcaaaga agtttgtcct tctgctattg atcctgaaga tggggaaaaa
     1501 aagaatcaag tgatgattca tggaattgag ccaatgttgg aaacacctct gcagtggctg
     1561 agtgaacatc tgagctaccc ggataatttt cttcatatta gtatcatccc acagccaaca
     1621 gattgaggta ccgcgggccc gggatccacc ggatctagat aactgatcat aatcagccat
     1681 acc
//
