LOCUS       sequence-84197-e        2396 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Membrane Receptor, Excitation: 487, Emission: 509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2396)
  TITLE     Davidson mEmerald
REFERENCE   2  (bases 1 to 2396)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEmerald-ATG13-C-18
FEATURES             Location/Qualifiers
     source          1..2396
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      22..31
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(28..30,31..33,34..744)
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(73..94)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(334..353)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     681..702
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gatccgctag cgctaccggt cgccaccatg gtgagcaagg gcgaggagct gttcaccggg
       61 gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc
      121 ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc
      181 ggcaagctgc ccgtgccctg gcccaccctc gtgaccacct tgacctacgg cgtgcagtgc
      241 ttcgcccgct accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa
      301 ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc
      361 gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc
      421 aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaaggtc
      481 tatatcaccg ccgacaagca gaagaacggc atcaaggtga acttcaagac ccgccacaac
      541 atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac
      601 ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccaagct gagcaaagac
      661 cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact
      721 ctcggcatgg acgagctgta caagtccgga ctcagatcta gtagcggagg tagcgcatcc
      781 ggcggaagcg gaggaagcga aactgatctc aattcccagg acagaaagga cctggacaag
      841 tttattaaat tttttgccct caagactgtc caagtgattg tccaggctcg gcttggtgaa
      901 aagatttgca ctcgttcatc atcttctcca acgggttcag attggttcaa cttagcaatc
      961 aaagacatcc cagaggttac acatgaagca aagaaggcac tggcaggaca gctgcctgca
     1021 gtcgggaggt ccatgtgtgt ggagatttca cttaagactt ctgagggaga ttccatggag
     1081 ctggaaatat ggtgtcttga aatgaatgaa aagtgtgata aagaaatcaa agtttcctac
     1141 acggtgtaca acagactgtc attgctgctg aagtcccttc ttgctataac tagggtgaca
     1201 ccagcctata ggctctccag gaaacaaggg catgaatatg tcatattata caggatatat
     1261 tttggagaag ttcagctgag tggcttagga gaaggcttcc agacagttcg tgttgggaca
     1321 gtgggcaccc ctgtgggcac catcactctt tcttgtgctt acagaattaa cttggcattc
     1381 atgtctacca ggcaatttga gaggacccca cctatcatgg ggattattat tgatcacttt
     1441 gtggaccgtc cctatcccag ctcctctccc atgcacccct gcaattacag aactgctggt
     1501 gaggacactg gagtaatata cccgtctgta gaagactctc aagaagtgtg taccacctct
     1561 ttttccacct ccccaccatc ccagctctca agctctcgcc tttcctatca gcctgctgcc
     1621 ctgggcgttg gatcagctga cctggcttat ccagtagtgt ttgctgctgg cttaaatgct
     1681 acacaccctc accagctgat ggttcctggg aaggaaggtg gggtacccct tgctcccaac
     1741 cagcctgtcc atggtaccca ggctgaccag gagagactgg caacctgcac cccttctgac
     1801 agaacccact gtgctgccac accctccagt agtgaggata ctgaaaccgt atcaaacagc
     1861 agtgagggac gggcctcccc tcacgatgtc ttggagacca tctttgtccg aaaagtgggg
     1921 gcttttgtca acaaacccat taaccaggtg accctgacga gtttggatat accctttgcc
     1981 atgtttgctc ccaagaattt ggagctggag gataccgatc caatggtgaa tcctccagat
     2041 tccccagaga ctgaatctcc tctccagggc agcctgcact cagatggctc cagcgggggc
     2101 agcagtggca atacccatga tgactttgtt atgatagact ttaaaccagc tttttctaaa
     2161 gatgacattc ttccgatgga cctggggacc ttctatcggg agtttcagaa cccacctcag
     2221 ctgagcagcc tctccataga tattggagca cagtccatgg ctgaagactt ggactcatta
     2281 ccagagaagc tggctgtgca tgagaagaat gtccgcgagt ttgatgcctt tgtggaaacc
     2341 ctgcagtaag gatccaccgg atctagataa ctgatcataa tcagccatac caacat
//
