LOCUS       sequence-84198-e        2387 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Membrane Receptor, Excitation: 487, Emission: 509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2387)
  TITLE     Davidson mEmerald
REFERENCE   2  (bases 1 to 2387)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEmerald-ATG13-N-18
FEATURES             Location/Qualifiers
     source          1..2387
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      8..17
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      1613..1622
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1619..1621,1622..1624,1625..2338)
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1664..1685)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1925..1944)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2272..2293
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gctaggcgcc accatggaaa ctgatctcaa ttcccaggac agaaaggacc tggacaagtt
       61 tattaaattt tttgccctca agactgtcca agtgattgtc caggctcggc ttggtgaaaa
      121 gatttgcact cgttcatcat cttctccaac gggttcagat tggttcaact tagcaatcaa
      181 agacatccca gaggttacac atgaagcaaa gaaggcactg gcaggacagc tgcctgcagt
      241 cgggaggtcc atgtgtgtgg agatttcact taagacttct gagggagatt ccatggagct
      301 ggaaatatgg tgtcttgaaa tgaatgaaaa gtgtgataaa gaaatcaaag tttcctacac
      361 ggtgtacaac agactgtcat tgctgctgaa gtcccttctt gctataacta gggtgacacc
      421 agcctatagg ctctccagga aacaagggca tgaatatgtc atattataca ggatatattt
      481 tggagaagtt cagctgagtg gcttaggaga aggcttccag acagttcgtg ttgggacagt
      541 gggcacccct gtgggcacca tcactctttc ttgtgcttac agaattaact tggcattcat
      601 gtctaccagg caatttgaga ggaccccacc tatcatgggg attattattg atcactttgt
      661 ggaccgtccc tatcccagct cctctcccat gcacccctgc aattacagaa ctgctggtga
      721 ggacactgga gtaatatacc cgtctgtaga agactctcaa gaagtgtgta ccacctcttt
      781 ttccacctcc ccaccatccc agctctcaag ctctcgcctt tcctatcagc ctgctgccct
      841 gggcgttgga tcagctgacc tggcttatcc agtagtgttt gctgctggct taaatgctac
      901 acaccctcac cagctgatgg ttcctgggaa ggaaggtggg gtaccccttg ctcccaacca
      961 gcctgtccat ggtacccagg ctgaccagga gagactggca acctgcaccc cttctgacag
     1021 aacccactgt gctgccacac cctccagtag tgaggatact gaaaccgtat caaacagcag
     1081 tgagggacgg gcctcccctc acgatgtctt ggagaccatc tttgtccgaa aagtgggggc
     1141 ttttgtcaac aaacccatta accaggtgac cctgacgagt ttggatatac cctttgccat
     1201 gtttgctccc aagaatttgg agctggagga taccgatcca atggtgaatc ctccagattc
     1261 cccagagact gaatctcctc tccagggcag cctgcactca gatggctcca gcgggggcag
     1321 cagtggcaat acccatgatg actttgttat gatagacttt aaaccagctt tttctaaaga
     1381 tgacattctt ccgatggacc tggggacctt ctatcgggag tttcagaacc cacctcagct
     1441 gagcagcctc tccatagata ttggagcaca gtccatggct gaagacttgg actcattacc
     1501 agagaagctg gctgtgcatg agaagaatgt ccgcgagttt gatgcctttg tggaaaccct
     1561 gcagagcagc ggtggagcaa gcgcagccag tggtagcgcg gatccaccgg tcgccaccat
     1621 ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg
     1681 cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg
     1741 caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct
     1801 cgtgaccacc ttgacctacg gcgtgcagtg cttcgcccgc taccccgacc acatgaagca
     1861 gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt
     1921 caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt
     1981 gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa
     2041 gctggagtac aactacaaca gccacaaggt ctatatcacc gccgacaagc agaagaacgg
     2101 catcaaggtg aacttcaaga cccgccacaa catcgaggac ggcagcgtgc agctcgccga
     2161 ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta
     2221 cctgagcacc cagtccaagc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct
     2281 gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagtaagc
     2341 ggccgcgact ctagatcata atcagccata ccacatttgt agaagtt
//
