LOCUS       sequence-84199-e        2350 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Membrane Receptor, Excitation: 487, Emission: 509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2350)
  TITLE     Davidson mEmerald
REFERENCE   2  (bases 1 to 2350)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEmerald-ATG14-C-18
FEATURES             Location/Qualifiers
     source          1..2350
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      25..34
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(31..33,34..36,37..747)
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(76..97)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(337..356)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     684..705
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 tcagatccgc tagcgctacc ggtcgccacc atggtgagca agggcgagga gctgttcacc
       61 ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg
      121 tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc
      181 accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccttgaccta cggcgtgcag
      241 tgcttcgccc gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc
      301 gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc
      361 gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac
      421 ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa cagccacaag
      481 gtctatatca ccgccgacaa gcagaagaac ggcatcaagg tgaacttcaa gacccgccac
      541 aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc
      601 gacggccccg tgctgctgcc cgacaaccac tacctgagca cccagtccaa gctgagcaaa
      661 gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc
      721 actctcggca tggacgagct gtacaagtcc ggactcagat ctggcagcgg tggaggcagc
      781 gcatccggcg gaagcggaag cgcgtctccc agtgggaagg gagcccgggc gctggaggct
      841 cctggctgcg ggccccggcc gctcgcccgg gacctggtgg actccgtgga cgatgcggag
      901 gggctgtacg tggctgtgga gcgctgcccg ctgtgcaaca ctacccgccg gcggctgacc
      961 tgcgccaaat gcgttcagag cggcgatttc gtctacttcg acggccgcga ccgggagagg
     1021 tttatcgaca agaaggaaag gttaagccga cttaagagca agcaagaaga atttcagaaa
     1081 gaagtgttaa aagctatgga aggaaaatgg ataacagatc agttgagatg gaaaataatg
     1141 tcctgcaaga tgaggattga acagttaaaa caaacaatat gtaaaggaaa tgaagaaatg
     1201 gagaaaaatt ctgaaggcct tctcaaaacc aaggaaaaga atcagaagct ttacagtcga
     1261 gcacaacggc accaagagaa aaaggagaag attcagaggc ataatcgcaa acttggtgac
     1321 ctggtagaaa aaaagaccat tgacttaaga agtcattatg agcgtctggc aaatcttcga
     1381 cgatcccata tattagagct cacctctgtc atttttccaa tcgaggaagt aaagacgggt
     1441 gtgagagacc ccgcagatgt gtcttcagag agtgacagtg ccatgacctc cagcactgtg
     1501 agcaagcttg ctgaagcccg gaggacaact tacctctcag gacgatgggt ctgtgacgat
     1561 cacaacggag acaccagcat tagcattaca gggccttgga ttagcctccc taacaatggg
     1621 gactactctg cctactacag ctgggtggag gagaagaaaa caacccaggg gcctgacatg
     1681 gagcagagta accctgccta caccatcagt gctgcgctgt gctatgcaac tcagctggtc
     1741 aacattctgt ctcatatact tgatgtaaat cttcccaaaa agctctgcaa cagtgaattt
     1801 tgtggcgaaa atctaagcaa gcagaaattt actcgagcag tgaagaaact gaatgcaaat
     1861 attctttacc tttgtttttc tcagcatgta aatttagatc aattacaacc actgcatacc
     1921 ctcaggaatc taatgtacct ggtcagtcca agctctgaac acctaggcag gtcagggccc
     1981 tttgaagtac gagcagacct tgaggagtcc atggaatttg tggatcccgg agttgctgga
     2041 gaatcagatg agagcggaga tgagcgcgtc agcgatgaag aaaccgacct gggcacagac
     2101 tgggagaact tgcctagtcc ccggttttgt gatatccctt cccagtctgt ggaagtctcc
     2161 cagagtcaga gcacccaggc gtccccaccc atcgcgagca gcagtgcagg tgggatgatc
     2221 tcctctgcag cagcctcggt gacctcctgg tttaaagctt acactggaca ccgttaagaa
     2281 ttctgcagtc gacggtaccg cgggcccggg atccaccgga tctagataac tgatcataat
     2341 cagccatacc
//
