LOCUS       sequence-84200-e        2613 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Membrane Receptor, Excitation: 487, Emission: 509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2613)
  TITLE     Davidson mEmerald
REFERENCE   2  (bases 1 to 2613)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEmerald-ATG16-C-18
FEATURES             Location/Qualifiers
     source          1..2613
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      26..35
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(32..34,35..37,38..748)
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(77..98)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(338..357)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     685..706
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gtcagatccg ctagcgctac cggtcgccac catggtgagc aagggcgagg agctgttcac
       61 cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt
      121 gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac
      181 caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accttgacct acggcgtgca
      241 gtgcttcgcc cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc
      301 cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg
      361 cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga
      421 cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa
      481 ggtctatatc accgccgaca agcagaagaa cggcatcaag gtgaacttca agacccgcca
      541 caacatcgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg
      601 cgacggcccc gtgctgctgc ccgacaacca ctacctgagc acccagtcca agctgagcaa
      661 agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat
      721 cactctcggc atggacgagc tgtacaagtc cggactcaga tctcgagctc aagcttcggg
      781 agatagtggt gggagcggtt catcgtcggg cctgcgcgcc gcagactttc cccgctggaa
      841 gcgtcacatc gcggaggaac tgaggcgccg ggaccgactg cagaggcagg cgttcgagga
      901 gatcattctg cagtatacca agttgctgga aaagtcagat cttcattcag tattgaccca
      961 gaaactacaa gcagaaaagc atgacatgcc aaataggcat gaaataagtc ctggacatga
     1021 tggtgcgtgg aatgatagtc aactacaaga aatggcccag ttgaggatca aacaccagga
     1081 agagctgacc gaactgcaca agaagcgtgg ggagttagct cagttggtga ttgacctgaa
     1141 caaccaaatg cagcagaagg acaaggagat acagatgaat gaagcaaaga tttcggagta
     1201 tttacagacc atctctgacc tggagacaaa ctgcctggac ctgcgcacca aactgcagga
     1261 cctcgaggta gccaaccaga ccctgaagga tgagtatgac gccctgcaga ttacttttac
     1321 tgccctagaa gagaaactga ggaaaactac tgaggagaac caggaactgg tcaccagatg
     1381 gatggctgag aaggcccaag aagccaatcg cctcaatgca gagaatgaga aggactccag
     1441 gaggcgtcaa gcacggctgc agaaggagct tgcagaagca gcaaaggaac ctctacctgt
     1501 tgaacaggat gatgacattg aagtcattgt ggatgagacc tcagaccaca cagaagagac
     1561 ctctcccgtc cgagctgtca gcagagcagc tacgagacgc tctgtctctt ccatcccagt
     1621 cccccaggat atcatggaca ctcatcctgc ttctggtaaa gatgtgagag tcccaactac
     1681 tgcctcgtat gtcttcgatg cgcatgacgg agaggtcaac gcagtgcagt tcagtccagg
     1741 ctcccggttg ctggccactg gaggcatgga ccgcagggtg aaactttggg aagcattcgg
     1801 agataaatgt gaattcaagg gctccctgtc tggcagtaat gctggaatta caagcattga
     1861 atttgatagt gctggagctt acctattagc agcttcaaat gattttgcaa gccgaatctg
     1921 gactgtggat gattatcgat tacggcacac actcacaggc cacagcggga aagtcctctc
     1981 tgccaagttc ctgctggaca atgcacggat tgtctcagga agtcacgacc ggaccctcaa
     2041 actctgggat ctccgcagca aagtctgcat aaaaacagtg tttgcaggat ccagctgcaa
     2101 tgacattgtt tgcactgaac aatgtgtaat gagtggacat tttgacaaga aaattcgttt
     2161 ctgggatatc cggtcagaga gtgtggtccg agagatggaa ctgttaggga agatcactgc
     2221 tctggaccta aaccctgaga gaactgagct cctgagctgc tcccgtgatg acctgctaaa
     2281 agtcatcgac ctccggacaa atgcagtcaa acagacattc agtgcgcctg gattcaaatg
     2341 cggctctgac tggacccggg ttgtcttcag ccctgatggc agttacgtgg cagcaggctc
     2401 agccgagggt tctctttatg tctggagtgt gctgacaggg aaagtggaga aggttctttc
     2461 aaaacagcac agctcttcta tcaatgcggt ggcgtgggcc ccctcgggct tacatgttgt
     2521 cagtgtggac aaaggaagca gagctatgct gtgggcacag ccttgaggta ccgcgggccc
     2581 gggatccacc ggatctagat aactgatcat aat
//
