LOCUS       sequence-84202-e        1459 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Cilium, Excitation: 487, Emission: 509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1459)
  TITLE     Davidson mEmerald
REFERENCE   2  (bases 1 to 1459)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEmerald-B9D1-N-18
FEATURES             Location/Qualifiers
     source          1..1459
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      31..40
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      697..706
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(703..705,706..708,709..1422)
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(748..769)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1009..1028)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1356..1377
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 ccgctagcgc taccggactc agatctcgag gccaccatgg ctgcagcaag tcccagcgtc
       61 ttcctgctca tgatcaccgg gcaggtagag agcgcacagt ttccagagta tgatgacctc
      121 tactgcaagt actgctttgt gtatggccag gactgggccc caacagcggg tctggaggag
      181 gggatctcac agatagcatc taagagccaa gatgtacggc aagcactggt gtggaacttc
      241 cccatcgatg tgacctttaa aagtaccaac ccctatggct ggccacagat tgtactcagt
      301 gtctatgggc cggatgtgtt tgggaatgat gtcgtccgag gctatggagc agtgcatgtg
      361 cccctctctc caggacggca caaaaggacc atccccatgt ttgtgccaga gtctacatct
      421 acactacaga agttcacaag ctggttcatg ggacggcgac ccgagtacac agaccccaag
      481 gtggtggccc agggtgaagg ccgggaagtg actcgtgttc gctctcaggg atttgttacc
      541 ctcctcttca atgtggtgac caaggacatg aagaagctgg gctatgatac tgggcctgtg
      601 gacacacaag gagtcctggg tcctagcctg ccacagggca acccacaaag tcgcggaggc
      661 agtggtgggg taccgcgggc ccgggatcca ccggtcgcca ccatggtgag caagggcgag
      721 gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac
      781 aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag
      841 ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccttgacc
      901 tacggcgtgc agtgcttcgc ccgctacccc gaccacatga agcagcacga cttcttcaag
      961 tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac
     1021 tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg
     1081 aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac
     1141 aacagccaca aggtctatat caccgccgac aagcagaaga acggcatcaa ggtgaacttc
     1201 aagacccgcc acaacatcga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac
     1261 acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag cacccagtcc
     1321 aagctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc
     1381 gccgccggga tcactctcgg catggacgag ctgtacaagt aagcggccgc gactctagat
     1441 cataatcagc cataccaca
//
