LOCUS       sequence-84205-e        3200 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Tight Junctions, Excitation: 487, Emission: 509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3200)
  TITLE     Davidson mEmerald
REFERENCE   2  (bases 1 to 3200)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEmerald-Beta-Catenin-20
FEATURES             Location/Qualifiers
     source          1..3200
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      22..31
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(28..30,31..33,34..744)
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(73..94)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(334..353)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     681..702
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     regulatory      799..808
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             850..885
                     /codon_start=1
                     /product="nanobody epitope tag corresponding to amino acids
                     16-27 of human beta-catenin (Traenkle et al., 2015)"
                     /label=BC2 tag
                     /translation="PDRKAAVSHWQQ"
ORIGIN
        1 gatccgctag cgctaccggt cgccaccatg gtgagcaagg gcgaggagct gttcaccggg
       61 gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc
      121 ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc
      181 ggcaagctgc ccgtgccctg gcccaccctc gtgaccacct tgacctacgg cgtgcagtgc
      241 ttcgcccgct accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa
      301 ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc
      361 gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc
      421 aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaaggtc
      481 tatatcaccg ccgacaagca gaagaacggc atcaaggtga acttcaagac ccgccacaac
      541 atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac
      601 ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccaagct gagcaaagac
      661 cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact
      721 ctcggcatgg acgagctgta caagtccgga ctcagatctc gagctcaagc ttcgaattct
      781 gcagtcgacg gtaccgcggc caccatggct actcaagctg acctgatgga gttggacatg
      841 gccatggagc cggacagaaa agctgctgtc agccactggc agcagcagtc ttacttggat
      901 tctggaatcc attctggtgc caccaccaca gctccttccc tgagtggcaa gggcaaccct
      961 gaggaagaag atgttgacac ctcccaagtc ctttatgaat gggagcaagg cttttcccag
     1021 tccttcacgc aagagcaagt agctgatatt gacgggcagt atgcaatgac tagggctcag
     1081 agggtccgag ctgccatgtt ccctgagacg ctagatgagg gcatgcagat cccatccacg
     1141 cagtttgacg ctgctcatcc cactaatgtc cagcgcttgg ctgaaccatc acagatgttg
     1201 aaacatgcag ttgtcaattt gattaactat caggatgacg cggaacttgc cacacgtgca
     1261 attcctgagc tgacaaaact gctaaacgat gaggaccagg tggtagttaa taaagctgct
     1321 gttatggtcc atcagctttc caaaaaggaa gcttccagac atgccatcat gcgctcccct
     1381 cagatggtgt ctgccattgt acgcaccatg cagaatacaa atgatgtaga gacagctcgt
     1441 tgtactgctg ggactctgca caacctttct caccaccgcg agggcttgct ggccatcttt
     1501 aagtctggtg gcatcccagc gctggtgaaa atgcttgggt caccagtgga ttctgtactg
     1561 ttctacgcca tcacgacact gcataatctc ctgctccatc aggaaggagc taaaatggca
     1621 gtgcgcctag ctggtggact gcagaaaatg gttgctttgc tcaacaaaac aaacgtgaaa
     1681 ttcttggcta ttacaacaga ctgccttcag atcttagctt atggcaatca agagagcaag
     1741 ctcatcattc tggccagtgg tggaccccaa gccttagtaa acataatgag gacctacact
     1801 tatgagaagc ttctgtggac cacaagcaga gtgctgaaag tgctgtctgt ctgctctagc
     1861 aacaagccgg ccattgtaga agctggtggg atgcaggcac tggggcttca tctgacagac
     1921 ccaagtcagc gacttgttca aaactgtctt tggactctca gaaacctttc agatgcagcg
     1981 actaagcagg aagggatgga aggcctcctt gggactctag tgcagcttct gggttccgat
     2041 gatataaatg tggtcacctg tgcagctgga attctctcta acctcacttg caataattac
     2101 aaaaacaaga tgatggtgtg ccaagtgggt ggcatagagg ctcttgtacg caccgtcctt
     2161 cgtgctggtg acagggaaga catcactgag cctgccatct gtgctcttcg tcatctgacc
     2221 agccggcatc aggaagccga gatggcccag aatgccgttc gccttcatta tggactgcct
     2281 gttgtggtta aactcctgca cccaccatcc cactggcctc tgataaaggc aactgttgga
     2341 ttgattcgaa accttgccct ttgcccagca aatcatgcgc ctttgcggga acagggtgct
     2401 attccacgac tagttcagct gcttgtacga gcacatcagg acacccaacg gcgcacctcc
     2461 atgggtggaa cgcagcagca gtttgtggag ggcgtgcgca tggaggagat agtagaaggg
     2521 tgtactggag ctctccacat ccttgctcgg gacgttcaca accggattgt aatccgagga
     2581 ctcaatacca ttccattgtt tgtgcagttg ctttattctc ccattgaaaa tatccaaaga
     2641 gtagctgcag gggtcctctg tgaacttgct caggacaagg aggctgcaga ggccattgaa
     2701 gctgagggag ccacagctcc cctgacagag ttactccact ccaggaatga aggcgtggca
     2761 acatacgcag ctgctgtcct attccgaatg tctgaggaca agccacagga ttacaagaag
     2821 cggctttcag tcgagctgac cagttccctc ttcaggacag agccaatggc ttggaatgag
     2881 actgcagatc ttggactgga cattggtgcc cagggagaag cccttggata tcgccaggat
     2941 gatcccagct accgttcttt tcactctggt ggatacggcc aggatgcctt ggggatggac
     3001 cctatgatgg agcatgagat gggtggccac caccctggtg ctgactatcc agttgatggg
     3061 ctgcctgatc tgggacacgc ccaggacctc atggatgggc tgcccccagg tgatagcaat
     3121 cagctggcct ggtttgatac tgacctgtaa aagcttggat ccaccggatc tagataactg
     3181 atcataatca gccataccac
//
