LOCUS       sequence-84206-e        3550 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Chromatin Assembly Factor 1, Excitation: 487, 
            Emission: 509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3550)
  TITLE     Davidson mEmerald
REFERENCE   2  (bases 1 to 3550)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEmerald-CAF1-C-10
FEATURES             Location/Qualifiers
     source          1..3550
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      28..37
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(34..36,37..39,40..750)
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(79..100)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(340..359)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     687..708
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    2569..2580
                     /label=ZF binding site
                     /bound_moiety="CCR5TC zinc-finger domain"
                     /note="target sequence for the CCR5TC zinc-finger domain 
                     from the CCR5-224 (+) zinc-finger nuclease (Perez et al., 
                     2008; Gross et al., 2013)"
ORIGIN
        1 acgtcagatc cgctagcgct accggtcgcc accatggtga gcaagggcga ggagctgttc
       61 accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc
      121 gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc
      181 accaccggca agctgcccgt gccctggccc accctcgtga ccaccttgac ctacggcgtg
      241 cagtgcttcg cccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg
      301 cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc
      361 cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc
      421 gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta caacagccac
      481 aaggtctata tcaccgccga caagcagaag aacggcatca aggtgaactt caagacccgc
      541 cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc
      601 ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc caagctgagc
      661 aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg
      721 atcactctcg gcatggacga gctgtacaag tccggactca gatctggcag cggtggaggc
      781 ttggaggagc cggaggccgc gacgcggaca gccgcggccg tggattgcaa agacagaccc
      841 gggttcccag tgaagaggtt aatacaagcc cgcttgccct tcaaacgcct gaaccttgtt
      901 cccaaggaga aagttgagga agacacatca ccaaaagcag ctgtggaaag caaagtcccg
      961 gatctacaat tgtctttggg cacttttgaa agccagtgtc acacaggctc tcacgtaggc
     1021 ttgagtacaa agttggtcgg tggccagggc cccattgata gcttcttgag ggcaacaatc
     1081 aagccagtac cgagtgtggt cattatcgac ctgacggaga actgcagtga cattccagac
     1141 agccctgagg gccacagtga actaagtcct gacactgctg gtgtggtcac cacagtggaa
     1201 ggagctgcca agcagcagga gcactctgca gcagagctgt gtctcctaga gaccccttca
     1261 gacatcactt gtcacatgga agaggagcct gggagtccag gagacccaaa gaggacgggt
     1321 gactgtcagg ctggttcact acagagctgt ccggagctga ccccaggttc taggacatgc
     1381 cccaccaagg agttatcaag ctggagcaag gcaggggatc tcctatttat agagaaagtg
     1441 cctgtggtgg tgctagagga catcctggcc actaagccct ccattgcctc tcttcccatg
     1501 atgtccctgg ataggagtgt cacttcagag agtgaaatac tggagtcctg ccccgaggat
     1561 gactccatac tgagccactc ctccaccaac tcctcttctc caaccagctc tcctgaggga
     1621 ccatccacac ccccagaaca tcggggtggg agaagctccc cctccacccc agcctgcaga
     1681 gttgccaaga actttgtcaa aggctccaca gagaagggca ggagcaaact gcacagagat
     1741 agagaacagc agagggaaga gaaggaaaag ctgagggaag agatacgacg agccaaggag
     1801 gaggcacgga agaagaagga ggaggagaag gagctgaagg aaaaggagcg gagggaaaag
     1861 cgggagaaag atgagaagga gaaggcggag aagcagcggc taaaggagga aaaacgcaag
     1921 gagcggcagg aagctctgga ggctaaactg gaggagaaga ggaagaagga ggaagagaag
     1981 cggttacggg aggaagagaa gcggttacgg gaagaagaga agcgcattaa ggcagaaaag
     2041 gcagagatca ctcgattctt ccagaaaccg aagactccgc aggctcccaa gaccctggcc
     2101 ggttcctgtg gtaagtttgc cccctttgaa atcaaagaac acatggtgct ggctccacgg
     2161 tgccgagctg ccttggatca agacctgtgc gatcagctgg accagctcct gcagcagcag
     2221 agtgtggcaa gcaccttcct cagtgaccta aaaagccggc tgcccttgag atcggggccc
     2281 acccgggtct gtggccatga cacagacatc atgaacaggg atgtggtcat tgtggagagc
     2341 agcaaggtgg acggtgtgtc tgaaaggaag aagtttggcc gcatgaagct tctgcagttc
     2401 tcagagaacc accggccagc atactggggg acgtggaaca agaagacagc catcatccgc
     2461 ccccggaacc cctgggccca ggacaaagac cttcttgact atgaggtaga cagtgacgat
     2521 gagtgggaag aggaggaacc tggggagtct ttgtcccaca gtgaggggga tgaggatgac
     2581 gatgtggggg aagatgagga tgaggacgat ggcttctttg tgccccatgg gtacttatct
     2641 gaagatgaag gcgtgactga ggagtgtgca gaccctgaga accacaaagt acaccagaag
     2701 ctgaaggcca aggagtggga tgagctccta gccaagggca agcgctttcg tgtgctgcag
     2761 cctgtgcacg tgggatgtgt atgggcatca gaggcagcca actgcacgag ttccgacttg
     2821 aagctgctgc agcagttcac tgcatgcctg ctggatgtgg catcccctga tgagccagaa
     2881 cccggggcct ccaggaggga aaagagggac cagcatatcc tggcccagtt gcttccgctg
     2941 ctacatggca atgtgaatgg gagcaaggtg atcatccatg aattccagga gcaatgccgc
     3001 aggggcctgc tgacccttcc aagtcccacc ccacacctgc agatgcccaa cttggaggat
     3061 gctgttgctg tgccatccaa ggccaggctg aagcgcctta tctctgagaa ctctgcctat
     3121 gagaagcggc ccaacttccg tatgtgctgg tatgtgcacc cagaggtgct caagagcttt
     3181 ggccaggagt gcctgccagt gccctgccag tggacctaca tcaccaccat gccctcagca
     3241 cccagggaag acagtggcag tgcttctact gaggggcctg gccagagcac acctatgcta
     3301 ctcaagcgga agccagcggc caccatgtgc atcacgcagt ttatgaagaa gcggcgctat
     3361 gatggacagg ttgggtctgg ggacatggat ggcttccagg cagacacaga agaggatgaa
     3421 gaggatgaca cagactgtat gatcatagat gtgccagatg ttgggagtga cgtgtcagaa
     3481 gctcccatac ctgctcccac actttgcaag tgaggatcca ccggatctag ataactgatc
     3541 ataatcagcc
//
