LOCUS       sequence-84207-e        3539 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Chromatin Assembly Factor 1, Excitation: 487, 
            Emission: 509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3539)
  TITLE     Davidson mEmerald
REFERENCE   2  (bases 1 to 3539)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEmerald-CAF1-N-10
FEATURES             Location/Qualifiers
     source          1..3539
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    1809..1820
                     /label=ZF binding site
                     /bound_moiety="CCR5TC zinc-finger domain"
                     /note="target sequence for the CCR5TC zinc-finger domain 
                     from the CCR5-224 (+) zinc-finger nuclease (Perez et al., 
                     2008; Gross et al., 2013)"
     regulatory      2775..2784
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(2781..2783,2784..2786,2787..3500)
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2826..2847)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3087..3106)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3434..3455
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gatccgctag cgccaccatg ttggaggagc cggaggccgc gacgcggaca gccgcggccg
       61 tggattgcaa agacagaccc gggttcccag tgaagaggtt aatacaagcc cgcttgccct
      121 tcaaacgcct gaaccttgtt cccaaggaga aagttgagga agacacatca ccaaaagcag
      181 ctgtggaaag caaagtcccg gatctacaat tgtctttggg cacttttgaa agccagtgtc
      241 acacaggctc tcacgtaggc ttgagtacaa agttggtcgg tggccagggc cccattgata
      301 gcttcttgag ggcaacaatc aagccagtac cgagtgtggt cattatcgac ctgacggaga
      361 actgcagtga cattccagac agccctgagg gccacagtga actaagtcct gacactgctg
      421 gtgtggtcac cacagtggaa ggagctgcca agcagcagga gcactctgca gcagagctgt
      481 gtctcctaga gaccccttca gacatcactt gtcacatgga agaggagcct gggagtccag
      541 gagacccaaa gaggacgggt gactgtcagg ctggttcact acagagctgt ccggagctga
      601 ccccaggttc taggacatgc cccaccaagg agttatcaag ctggagcaag gcaggggatc
      661 tcctatttat agagaaagtg cctgtggtgg tgctagagga catcctggcc actaagccct
      721 ccattgcctc tcttcccatg atgtccctgg ataggagtgt cacttcagag agtgaaatac
      781 tggagtcctg ccccgaggat gactccatac tgagccactc ctccaccaac tcctcttctc
      841 caaccagctc tcctgaggga ccatccacac ccccagaaca tcggggtggg agaagctccc
      901 cctccacccc agcctgcaga gttgccaaga actttgtcaa aggctccaca gagaagggca
      961 ggagcaaact gcacagagat agagaacagc agagggaaga gaaggaaaag ctgagggaag
     1021 agatacgacg agccaaggag gaggcacgga agaagaagga ggaggagaag gagctgaagg
     1081 aaaaggagcg gagggaaaag cgggagaaag atgagaagga gaaggcggag aagcagcggc
     1141 taaaggagga aaaacgcaag gagcggcagg aagctctgga ggctaaactg gaggagaaga
     1201 ggaagaagga ggaagagaag cggttacggg aggaagagaa gcggttacgg gaagaagaga
     1261 agcgcattaa ggcagaaaag gcagagatca ctcgattctt ccagaaaccg aagactccgc
     1321 aggctcccaa gaccctggcc ggttcctgtg gtaagtttgc cccctttgaa atcaaagaac
     1381 acatggtgct ggctccacgg tgccgagctg ccttggatca agacctgtgc gatcagctgg
     1441 accagctcct gcagcagcag agtgtggcaa gcaccttcct cagtgaccta aaaagccggc
     1501 tgcccttgag atcggggccc acccgggtct gtggccatga cacagacatc atgaacaggg
     1561 atgtggtcat tgtggagagc agcaaggtgg acggtgtgtc tgaaaggaag aagtttggcc
     1621 gcatgaagct tctgcagttc tcagagaacc accggccagc atactggggg acgtggaaca
     1681 agaagacagc catcatccgc ccccggaacc cctgggccca ggacaaagac cttcttgact
     1741 atgaggtaga cagtgacgat gagtgggaag aggaggaacc tggggagtct ttgtcccaca
     1801 gtgaggggga tgaggatgac gatgtggggg aagatgagga tgaggacgat ggcttctttg
     1861 tgccccatgg gtacttatct gaagatgaag gcgtgactga ggagtgtgca gaccctgaga
     1921 accacaaagt acaccagaag ctgaaggcca aggagtggga tgagctccta gccaagggca
     1981 agcgctttcg tgtgctgcag cctgtgcacg tgggatgtgt atgggcatca gaggcagcca
     2041 actgcacgag ttccgacttg aagctgctgc agcagttcac tgcatgcctg ctggatgtgg
     2101 catcccctga tgagccagaa cccggggcct ccaggaggga aaagagggac cagcatatcc
     2161 tggcccagtt gcttccgctg ctacatggca atgtgaatgg gagcaaggtg atcatccatg
     2221 aattccagga gcaatgccgc aggggcctgc tgacccttcc aagtcccacc ccacacctgc
     2281 agatgcccaa cttggaggat gctgttgctg tgccatccaa ggccaggctg aagcgcctta
     2341 tctctgagaa ctctgcctat gagaagcggc ccaacttccg tatgtgctgg tatgtgcacc
     2401 cagaggtgct caagagcttt ggccaggagt gcctgccagt gccctgccag tggacctaca
     2461 tcaccaccat gccctcagca cccagggaag acagtggcag tgcttctact gaggggcctg
     2521 gccagagcac acctatgcta ctcaagcgga agccagcggc caccatgtgc atcacgcagt
     2581 ttatgaagaa gcggcgctat gatggacagg ttgggtctgg ggacatggat ggcttccagg
     2641 cagacacaga agaggatgaa gaggatgaca cagactgtat gatcatagat gtgccagatg
     2701 ttgggagtga cgtgtcagaa gctcccatac ctgctcccac actttgcaag tccggtagcg
     2761 cggatccacc ggtcgccacc atggtgagca agggcgagga gctgttcacc ggggtggtgc
     2821 ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg
     2881 gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc
     2941 tgcccgtgcc ctggcccacc ctcgtgacca ccttgaccta cggcgtgcag tgcttcgccc
     3001 gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg
     3061 tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga
     3121 agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg
     3181 acggcaacat cctggggcac aagctggagt acaactacaa cagccacaag gtctatatca
     3241 ccgccgacaa gcagaagaac ggcatcaagg tgaacttcaa gacccgccac aacatcgagg
     3301 acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg
     3361 tgctgctgcc cgacaaccac tacctgagca cccagtccaa gctgagcaaa gaccccaacg
     3421 agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca
     3481 tggacgagct gtacaagtaa gcggccgcga ctctagatca taatcagcca taccacatt
//
